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The effects of CPT-11/OCD NP treatment on the cell cycle of C26 cells were evaluated by quantifying the number of G2/M cells. After treatment with CPT-11/OCD NP (at 30 μg/mL of CPT-11) for 6 h. Cells were collected and washed with PBS, and then resuspended in PBS containing 0.03% Triton X-100, 200 mg/mL RNase A, and 50 μg/mL PI. ................
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