Exphem
Briefly, 293Tcells were incubated with 21 μg of pLV-[mir-locker] (Biosettia, San Diego, USA) or SEW-Fya-L-P2A-Fy-variant 7 μg of the vesicular stomatitis virus envelope glycoprotein envelope plasmid (pMD2.G) and 14 μg of the packaging plasmid pR8.94 for 12-16h. The following day, the medium was replaced with fresh medium. Supernatants were ... ................
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