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During hydration, the lipid layer was treated by sonication in 1M acetate, pH 2.3, for 1h at 30°C in the dark. Control nanoparticles were prepared in an identical manner but through lipid film hydration with sonication in 4 mM 4-(2-hydroxyethyl)-1-piperazine-ethanesulfonic acid (HEPES) buffer, pH … ................
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