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Nucleolar pattern . ... ,a haemoflagellate is used as a substrate for detecting Anti ds DNA and HEp2cells are used in detection of other ANA. The serum diluted 1/40 in phosphate buffer saline(PBS) was overlaid onto fixed HEp2 cells for 30 minutes at room temperature.Slides were washed twice for 5 minutes each with PBS overlaid with Flourescence ... ................
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