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Purified anti-IKKγ (NEMO) Antibody Total cell lysates (15 ?g protein) from SH-SY5Y, HeLa, NIH3T3 were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with 1 ?g/mL purified anti-IKKγ (NEMO) antibody (clone W16070A) (upper). Proteins were visualized using a goat anti-rat-IgG secondary antibody conjugated to HRP and chemiluminescence detection. Direct-Blot? HRP anti-β-actin antibody was used as a loading control (lower).Purified anti-IKKγ (NEMO) AntibodyHeLa cells were fixed with 4% paraformaldehyde (PFA) for fifteen minutes, permeabilized with 0.5% Triton X-100 for three minutes, and blocked with 5% FBS for one hour. Then the cells were intracellularly stained with 2 ?g/mL of purified anti-IKKγ (NEMO) antibody (clone W16070A) overnight at 4°C followed by Alexa Fluor? 594 (red) conjugated goat anti-rat IgG for one hour at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective. ................
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