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Nov 08, 2016 · Gene-specific primers were used to quantify the resulting first-strand cDNA by qPCR using the SYBR green PCR master mix (Life Technologies) on an AB7900HT thermocycler (Applied Biosystems). The ∆∆CT method was applied to determine relative mRNA quantities, normalizing mRNA across samples to β-actin or gapdh transcript levels. ................
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