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Prepare stocks: 5% Mucin (0.5g in 10mL of PBS) autoclave. 10 mg/ml salmon sperm DNA (100mg in 10 ml, this must be heated to almost boiling to dissolve, best is around 85C for a few hours) 44mg/mL KCl (4.4g/100ml) 100mg/mL NaCl (10g in 100mL) 1 mg/mL ferritin (or ferritin) filter sterilize. To make 20 mL. 8mL of mucin (2%) 1mL of KCl . 1mL NaCl. 100ul egg yolk emulsion. Add:2.8 mL of DNA. 0.06 ... ................
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