Cambridge International AS & A Level - GCE Guide
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Cambridge International AS & A Level
BIOLOGY Paper 1 Multiple Choice
9700/12 February/March 2020
1 hour
You must answer on the multiple choice answer sheet.
You will need: Multiple choice answer sheet Soft clean eraser Soft pencil (type B or HB is recommended)
INSTRUCTIONS ? There are forty questions on this paper. Answer all questions. ? For each question there are four possible answers A, B, C and D. Choose the one you consider correct
and record your choice in soft pencil on the multiple choice answer sheet. ? Follow the instructions on the multiple choice answer sheet. ? Write in soft pencil. ? Write your name, centre number and candidate number on the multiple choice answer sheet in the
spaces provided unless this has been done for you. ? Do not use correction fluid. ? Do not write on any bar codes. ? You may use a calculator.
INFORMATION ? The total mark for this paper is 40. ? Each correct answer will score one mark. A mark will not be deducted for a wrong answer. ? Any rough working should be done on this question paper.
IB20 03_9700_12/4RP ? UCLES 2020
This document has 16 pages. Blank pages are indicated.
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2 1 The diagram shows an eyepiece graticule and cell viewed through a microscope. When the
eyepiece graticule was calibrated at this magnification, the whole length of the graticule shown covered 12 divisions of a stage micrometer scale. There were 100 divisions in 10 mm of the stage micrometer.
0 10 20 30 40 50 60 70 80 90 100
What is the actual length of the cell?
A 2.5 ?m
B 3.6 ?m
C 360 ?m
D 3 mm
2 Which cell structures can form vesicles?
cell structure
cell surface membrane
endoplasmic reticulum
Golgi body
A
key
B
= can form vesicles
C
= cannot form vesicles
D
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3 The diagram shows three circles, 1, 3 and 5, representing chloroplasts, mitochondria and typical prokaryotes.
circle 1
circle 3
circle 5
2
4
Which row correctly identifies the three circles and some of the structures that are shared between them?
circle 1
2
circle 3
4
circle 5
A chloroplasts circular DNA mitochondria 80S ribosomes prokaryotes B chloroplasts 80S ribosomes mitochondria circular DNA prokaryotes C prokaryotes circular DNA mitochondria circular DNA chloroplasts D prokaryotes 70S ribosomes chloroplasts 80S ribosomes mitochondria
4 Which cell structures contain RNA? 1 centrioles 2 mitochondria 3 nucleus 4 ribosomes
A 1, 2, 3 and 4 B 1 and 2 only C 2, 3 and 4 only D 3 and 4 only
5 It is possible for a bacterium to synthesise a eukaryotic protein. This involves introducing a eukaryotic gene into the bacterial DNA. The eukaryotic gene is then translated by the bacterium. What explains why a bacterial cell can produce a eukaryotic protein but cannot produce a eukaryotic glycoprotein? A Bacteria do not have rough endoplasmic reticulum. B Bacteria do not have a nuclear envelope. C Bacteria do not have mitochondria. D Bacteria do not have Golgi bodies.
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4
6 Which structures are found in typical prokaryotic cells and also in typical plant cells? A cell walls B histones C telomeres D tonoplasts
7 The molecule shown is a polymer of reducing sugars.
CH2OH O
OH
O
HO
OH
CH2OH O
OH
O
OH
CH2OH O
OH
O
OH
CH2OH O
OH OH
OH
Which procedures could be carried out to show that this molecule is a polymer of reducing sugars?
1 Add hydrolytic enzyme and then heat with Benedict's solution. 2 Dissolve in water, neutralise and then heat with Benedict's solution. 3 Boil with hydrochloric acid, neutralise and then heat with Benedict's solution.
A 1, 2 and 3
B 1 and 2 only C 1 and 3 only D 2 and 3 only
8 Which statement about biological molecules is correct? A Amylopectin, amylose and cellulose are all polymers. B Amylose, cellulose and glucose are all macromolecules. C Cellulose, glucose and starch are all monomers. D Sucrose, starch and amylopectin are all polysaccharides.
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9 Sugars with a ring structure can also have a linear structure.
H
CO
H C OH
HO C H
H C OH
H C OH
CH2OH
Which of these sugar molecules could be represented by the linear structure in the diagram?
1 glucose 2 ribose 3 sucrose
A 1 and 2
B 2 and 3
C 1 only
D 2 only
10 Which statements about phospholipids and triglycerides are correct?
1 They both contain ester bonds. 2 They both have three fatty acid chains per molecule. 3 They both may have saturated and unsaturated fatty acid chains. 4 They are both used only as storage molecules.
A 1, 2 and 3
B 1 and 3 only C 2, 3 and 4
D 2 and 4 only
11 The diagrams show three examples of different bonds.
bond 1 NH O C
O bond 2 CN
H
bond 3 CH2 S S CH2
Which bonds hold the secondary structure of proteins together? A 1, 2 and 3 B 1 and 2 only C 1 only D 2 and 3 only
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12 Hydrogen bonding explains many of the properties of water, including the high latent heat of vapourisation and high specific heat capacity.
For which processes in plants is hydrogen bonding in water important on hot sunny days? 1 preventing denaturation of enzymes in leaves 2 reducing water loss by evaporation 3 allowing leaves to cool down quickly at night 4 holding the column of water in xylem vessels together
A 1, 2, 3 and 4 B 1, 2 and 4 only C 1, 3 and 4 only D 2 and 3 only
13 What is the most appropriate set of controls to use in an investigation into the rate of an enzyme-catalysed reaction over a range of temperatures from 25 ?C to 65 ?C?
A enzyme and substrate at all temperatures tested B enzyme and boiled substrate at all temperatures tested C boiled enzyme only at all temperatures tested D substrate only at all temperatures tested
14 An investigation is carried out with an enzyme at its optimum temperature and pH. The rate of the enzyme reaction is measured at different substrate concentrations. The investigation is repeated in the presence of a competitive inhibitor. How will the results with the competitive inhibitor be different from the original results?
Km with competitive Vmax with competitive
inhibitor
inhibitor
A
higher
B
higher
C
the same
D
the same
lower the same
lower the same
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15 Which description of cell surface membrane permeability is correct?
A An increase in the concentration of cholesterol molecules in the cell surface membrane can increase its permeability to hydrophilic substances.
B Cell surface membrane permeability to large hydrophilic molecules is high and can be increased by membrane transport proteins involved in facilitated diffusion.
C The permeability of the cell surface membrane to ions increases as the proportion of saturated fatty acid chains in the phospholipids increases.
D Without the presence of carrier and channel membrane proteins, the cell surface membrane has a low permeability to large polar molecules.
16 Which transport mechanism within a cell can occur in the absence of membranes? A active transport B diffusion C facilitated diffusion D osmosis
17 A student measured the time taken for complete diffusion of a dye into agar blocks of different sizes.
The results are shown in the table.
size of agar block / mm ? mm ? mm
5 ? 5 ? 5 10 ? 10 ? 10 15 ? 15 ? 15 5 ? 10 ? 15
time for diffusion / s
6.2 16.1 34.5
What is the predicted time for complete diffusion of the dye into the agar block measuring 5 mm ? 10 mm ? 15 mm?
A 6.2 s B 16.1 s C 34.5 s D more than 34.5 s
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18 A plant cell with a water potential of ?600 kPa was placed in a solution with a water potential of ?410 kPa for 10 minutes.
Which row is correct?
net movement of water
water potential of cell
effect on cell
A
into cell
becomes higher
becomes turgid
B
into cell
becomes lower
bursts
C
out of cell
becomes higher
swells
D
out of cell
becomes lower becomes plasmolysed
19 Which cells contain telomeres that are longer than those in a helper T-lymphocyte secreting cytokines?
1 bone marrow stem cells 2 mature red blood cells 3 activated memory B-lymphocytes
A 1 and 2
B 1 and 3
C 2 only
D 3 only
20 In which stage of the cell cycle are telomeres needed to prevent the loss of genes? A prophase B cytokinesis C G1 phase D S phase
21 Which row shows part of the correct sequence of mitosis?
A
chromosomes condense
chromosomes line up at the
equator
nuclear envelope disappears
spindle fibres shorten
B
chromosomes line up at the
equator
spindle fibres shorten
chromosomes condense
nuclear envelope reappears
C
chromosomes line up at the
equator
spindle fibres shorten
nuclear envelope reappears
chromosomes uncoil
D
chromosomes uncoil
nuclear envelope disappears
spindle fibres shorten
chromosomes line up at the
equator
? UCLES 2020
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