Addgene



All strains are derivatives of BL21(DE3) (genotype F– ompT gal dcm lon hsdSB(rB–mB–) λ(DE3 [lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB+]K-12(λS))The strains lack the coding sequence of one or more abundant outer membrane proteins (OmpA, OmpC, OmpF, LamB)Knock-outsStrainGenes knocked outGenotypeSingle knock-outsBL21ΔAompAF– ompT gal dcm lon hsdSB(rB–mB–) λ(DE3 [lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB+]K-12(λS) ΔompABL21ΔBlamBF– ompT gal dcm lon hsdSB(rB–mB–) λ(DE3 [lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB+]K-12(λS) ΔlamBBL21ΔCompCF– ompT gal dcm lon hsdSB(rB–mB–) λ(DE3 [lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB+]K-12(λS) ΔompCBL21ΔFompFF– ompT gal dcm lon hsdSB(rB–mB–) λ(DE3 [lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB+]K-12(λS) ΔompFDouble knock-outsBL21ΔABompA, lamBF– ompT gal dcm lon hsdSB(rB–mB–) λ(DE3 [lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB+]K-12(λS) ΔompA ΔlamBBL21ΔACompA, ompCF– ompT gal dcm lon hsdSB(rB–mB–) λ(DE3 [lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB+]K-12(λS) ΔompA ΔompCBL21ΔAFompA, ompFF– ompT gal dcm lon hsdSB(rB–mB–) λ(DE3 [lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB+]K-12(λS) ΔompA ΔompFBL21ΔBClamB, ompCF– ompT gal dcm lon hsdSB(rB–mB–) λ(DE3 [lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB+]K-12(λS) ΔompC ΔlamBBL21ΔBFlamB, ompFF– ompT gal dcm lon hsdSB(rB–mB–) λ(DE3 [lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB+]K-12(λS) ΔompF ΔlamBBL21ΔCFompC, ompFF– ompT gal dcm lon hsdSB(rB–mB–) λ(DE3 [lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB+]K-12(λS) ΔompC ΔompFTriple knock-outsBL21ΔABCompA, lamB, ompCF– ompT gal dcm lon hsdSB(rB–mB–) λ(DE3 [lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB+]K-12(λS) ΔompA ΔompC ΔlamBBL21ΔABFompA, lamB, ompFF– ompT gal dcm lon hsdSB(rB–mB–) λ(DE3 [lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB+]K-12(λS) ΔompA ΔompF ΔlamBBL21ΔACFompA, ompC, ompFF– ompT gal dcm lon hsdSB(rB–mB–) λ(DE3 [lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB+]K-12(λS) ΔompA ΔompC ΔompFBL21ΔBCFlamB, ompC, ompFF– ompT gal dcm lon hsdSB(rB–mB–) λ(DE3 [lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB+]K-12(λS) ΔlamB ΔompC ΔompFQuadruple knock outBL21ΔABCFompA, lamB, ompC, ompFF– ompT gal dcm lon hsdSB(rB–mB–) λ(DE3 [lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB+]K-12(λS) ΔompA ΔompC ΔompF ΔlamBPrimersompA locus: 648 + 649, expected size ~130 bpompC locus: 650 +651, expected size ~130 kbompF locus: 652 + 653, expected size ~130 bplamB locus: 646 + 647, expected size ~150 bpPrimer sequences:646: AAA AGA AAA GCA ATGACT CAG GAG ATA GAA TG647: GGT TTT GCT ATT ACCACC AGA TTT CCA TCT G648: ATTTTGGATGATAACGAGGCGCAAAAAATG649: GAACTTAAGCCTGCGGCTGAGTTAC650: GCA AAT AAA GGC ATA TAA CAG AGG GTT AAT AAC ATG651: ATA TCA ATC GAG ATT AGA ACT GGT AAA CCA GAC C652: aggtgtcataaaaaaaaccatgagggtaataaataatg653: gaggtgtgctattagaactggtaaacgatacc ................
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