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Supplementary Table 1. Bacterial Strains and Plasmids.Strain orPlasmidRelevant Genotype, Featuresor CharacteristicsSource or ReferenceStrainAMM05BW25113 ?ldtD::frtThis workAMM06BW25113 ?ldtE::frtThis workAMM07BW25113 ?ldtE::frt ?ldtD::frtThis workAMM10BB-3 ?ldtD::frt This workAMM11BB-3 ?ldtE::frt This workAMM12BB-3 ?ldtD::frt ?ldtE::frt This workAMM14BB-3 ?ldtA::frt ?ldtB::frt ?ldtC::frtThis workAMM24 BW25113 ?ldtF::frtThis workAMM25 BW25113 ?ldtD::frt ?ldtF::frt This workAMM26 BW25113 ?ldtE::frt ?ldtF::frt This workAMM28 BW25113 ?ldtD::frt ?ldtE::frt ?ldtF::frt This workAMM30BB-3 ?ldtF::frtThis workAMM31BB-3 ?ldtD::frt ?ldtF::frt This workAMM32BB-3 ?ldtE::frt ?ldtF::frt This workAMM33 BB-3 ?ldtA::frt ?ldtB::frt ?ldtC::frt ?ldtD::frt ?ldtE::frt ?ldtF::frt This workAMM34BB-3 ?ldtD::frt ?ldtE::frt ?ldtF::frt This workAMM36BW25113 ?rpoS:: frtThis workAMM51BW25113 ?mrcA::frtThis workAMM52BW25113 ?mrcB::frtThis workAMM53BW25113 ?dacA::frtThis workAMM54BW25113 ?dacC::frtThis workAMM55BW25113 ?lpoB::frtThis workAMM56BW25113 ?cpoB::frtThis workAMM60BB-3 ?mrcA::frtThis workAMM61BB-3 ?mrcB::frtThis workAMM62BB-3 ?dacA::frtThis workAMM63BB-3 ?dacC::frtThis workAMM64BB-3 ?lpoB::frtThis workAMM65BB-3 ?cpoB::frtThis workAMM83BW25113 ldtD-his::frtThis workAMM84BB-3 ldtD-hisThis workBB-3BW25113 Φ(kan araC araBplptC)1 ADDIN EN.CITE <EndNote><Cite><Author>Sperandeo</Author><Year>2006</Year><RecNum>5</RecNum><DisplayText>(Sperandeo et al., 2006)</DisplayText><record><rec-number>5</rec-number><foreign-keys><key app="EN" db-id="tzfezx99lprtaterppyvd2xyzdsvvrvedx2z" timestamp="1510914801">5</key><key app="ENWeb" db-id="">0</key></foreign-keys><ref-type name="Journal Article">17</ref-type><contributors><authors><author>Sperandeo, P.</author><author>Pozzi, C.</author><author>Deho, G.</author><author>Polissi, A.</author></authors></contributors><auth-address>Dipartimento di Scienze biomolecolari e Biotecnologie, Universita degli Studi di Milano, Milan, Italy.</auth-address><titles><title>Non-essential KDO biosynthesis and new essential cell envelope biogenesis genes in the Escherichia coli yrbG-yhbG locus</title><secondary-title>Res Microbiol</secondary-title></titles><periodical><full-title>Res Microbiol</full-title></periodical><pages>547-58</pages><volume>157</volume><number>6</number><edition>2006/06/13</edition><keywords><keyword>Aldose-Ketose Isomerases/biosynthesis/genetics</keyword><keyword>Arabinose/metabolism</keyword><keyword>Cloning, Molecular</keyword><keyword>Escherichia coli/genetics/metabolism/*physiology</keyword><keyword>Escherichia coli Proteins/*biosynthesis/genetics</keyword><keyword>Gene Expression Regulation, Bacterial</keyword><keyword>*Genes, Essential</keyword><keyword>Lipopolysaccharides/metabolism</keyword><keyword>Mutation</keyword><keyword>Open Reading Frames</keyword><keyword>Phosphoric Monoester Hydrolases/biosynthesis/genetics</keyword><keyword>Promoter Regions, Genetic</keyword><keyword>Sugar Acids/*metabolism</keyword></keywords><dates><year>2006</year><pub-dates><date>Jul-Aug</date></pub-dates></dates><isbn>0923-2508 (Print)
0923-2508 (Linking)</isbn><accession-num>16765569</accession-num><urls><related-urls><url>;(Sperandeo et al., 2006)BL21(DE3)F– ompT hsdSB(rB– mB–) gal dcm (DE3)NovagenBW25113lacIq rrnBT14 ΔlacZWJ16 hsdR514 ΔaraBADAH33 ΔrhaBADLD78 ADDIN EN.CITE <EndNote><Cite><Author>Datsenko</Author><Year>2000</Year><RecNum>60</RecNum><DisplayText>(Datsenko and Wanner, 2000)</DisplayText><record><rec-number>60</rec-number><foreign-keys><key app="EN" db-id="tzfezx99lprtaterppyvd2xyzdsvvrvedx2z" timestamp="1510920867">60</key></foreign-keys><ref-type name="Journal Article">17</ref-type><contributors><authors><author>Datsenko, K. A.</author><author>Wanner, B. L.</author></authors></contributors><auth-address>Department of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA.</auth-address><titles><title>One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products</title><secondary-title>Proc Natl Acad Sci U S A</secondary-title></titles><periodical><full-title>Proc Natl Acad Sci U S A</full-title></periodical><pages>6640-5</pages><volume>97</volume><number>12</number><edition>2000/06/01</edition><keywords><keyword>*Chromosomes, Bacterial</keyword><keyword>DNA Nucleotidyltransferases/metabolism</keyword><keyword>Escherichia coli/*genetics</keyword><keyword>*Integrases</keyword><keyword>Lac Operon</keyword><keyword>*Mutation</keyword><keyword>Operon</keyword><keyword>Plasmids</keyword><keyword>*Polymerase Chain Reaction</keyword><keyword>Recombinases</keyword><keyword>Recombination, Genetic</keyword></keywords><dates><year>2000</year><pub-dates><date>Jun 06</date></pub-dates></dates><isbn>0027-8424 (Print)
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ADDIN EN.CITE.DATA (Kuru et al., 2017)DH5α(argF-lac169) 80 dlacZ58(M15) glnV44(AS) λ- rfbD1 gyrA96 recA1 endA1 spoT1 thi-1 hsdR17 ADDIN EN.CITE <EndNote><Cite><Author>Hanahan</Author><Year>1983</Year><RecNum>82</RecNum><DisplayText>(Hanahan, 1983)</DisplayText><record><rec-number>82</rec-number><foreign-keys><key app="EN" db-id="tzfezx99lprtaterppyvd2xyzdsvvrvedx2z" timestamp="1511191986">82</key></foreign-keys><ref-type name="Journal Article">17</ref-type><contributors><authors><author>Hanahan, D.</author></authors></contributors><titles><title>Studies on transformation of Escherichia coli with plasmids</title><secondary-title>J Mol Biol</secondary-title></titles><periodical><full-title>J Mol Biol</full-title></periodical><pages>557-80</pages><volume>166</volume><number>4</number><edition>1983/06/05</edition><keywords><keyword>Cations</keyword><keyword>DNA, Superhelical</keyword><keyword>Dimethyl Sulfoxide/pharmacology</keyword><keyword>Dithiothreitol/pharmacology</keyword><keyword>Escherichia coli/drug effects/*genetics/growth & development</keyword><keyword>*Plasmids/drug effects</keyword><keyword>Species Specificity</keyword><keyword>*Transformation, Bacterial/drug effects</keyword></keywords><dates><year>1983</year><pub-dates><date>Jun 05</date></pub-dates></dates><isbn>0022-2836 (Print)
0022-2836 (Linking)</isbn><accession-num>6345791</accession-num><urls><related-urls><url>;(Hanahan, 1983)JW0732BW25113 ?cpoB::kan(Baba et al., 2006)JW0803BW25113 ?ldtB790::kan ADDIN EN.CITE <EndNote><Cite><Author>Baba</Author><Year>2006</Year><RecNum>72</RecNum><DisplayText>(Baba et al., 2006)</DisplayText><record><rec-number>72</rec-number><foreign-keys><key app="EN" db-id="tzfezx99lprtaterppyvd2xyzdsvvrvedx2z" timestamp="1511171842">72</key></foreign-keys><ref-type name="Journal Article">17</ref-type><contributors><authors><author>Baba, T.</author><author>Ara, T.</author><author>Hasegawa, M.</author><author>Takai, Y.</author><author>Okumura, Y.</author><author>Baba, M.</author><author>Datsenko, K. A.</author><author>Tomita, M.</author><author>Wanner, B. L.</author><author>Mori, H.</author></authors></contributors><auth-address>Institute for Advanced Biosciences, Keio University, Tsuruoka City, Yamagata, Japan.</auth-address><titles><title>Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection</title><secondary-title>Mol Syst Biol</secondary-title></titles><periodical><full-title>Mol Syst Biol</full-title></periodical><pages>2006 0008</pages><volume>2</volume><edition>2006/06/02</edition><keywords><keyword>Escherichia coli/*genetics</keyword><keyword>Gene Deletion</keyword><keyword>Internet</keyword><keyword>Mutation</keyword><keyword>*Organisms, Genetically Modified</keyword></keywords><dates><year>2006</year></dates><isbn>1744-4292 (Electronic)
1744-4292 (Linking)</isbn><accession-num>16738554</accession-num><urls><related-urls><url>;(Baba et al., 2006)JW0908BW25113 ?ldtD742::kan ADDIN EN.CITE <EndNote><Cite><Author>Baba</Author><Year>2006</Year><RecNum>72</RecNum><DisplayText>(Baba et al., 2006)</DisplayText><record><rec-number>72</rec-number><foreign-keys><key app="EN" db-id="tzfezx99lprtaterppyvd2xyzdsvvrvedx2z" timestamp="1511171842">72</key></foreign-keys><ref-type name="Journal Article">17</ref-type><contributors><authors><author>Baba, T.</author><author>Ara, T.</author><author>Hasegawa, M.</author><author>Takai, Y.</author><author>Okumura, Y.</author><author>Baba, M.</author><author>Datsenko, K. A.</author><author>Tomita, M.</author><author>Wanner, B. L.</author><author>Mori, H.</author></authors></contributors><auth-address>Institute for Advanced Biosciences, Keio University, Tsuruoka City, Yamagata, Japan.</auth-address><titles><title>Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection</title><secondary-title>Mol Syst Biol</secondary-title></titles><periodical><full-title>Mol Syst Biol</full-title></periodical><pages>2006 0008</pages><volume>2</volume><edition>2006/06/02</edition><keywords><keyword>Escherichia coli/*genetics</keyword><keyword>Gene Deletion</keyword><keyword>Internet</keyword><keyword>Mutation</keyword><keyword>*Organisms, Genetically Modified</keyword></keywords><dates><year>2006</year></dates><isbn>1744-4292 (Electronic)
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1744-4292 (Linking)</isbn><accession-num>16738554</accession-num><urls><related-urls><url>;(Baba et al., 2006)JW5820BW25113 ?ldtC775::kan ADDIN EN.CITE <EndNote><Cite><Author>Baba</Author><Year>2006</Year><RecNum>72</RecNum><DisplayText>(Baba et al., 2006)</DisplayText><record><rec-number>72</rec-number><foreign-keys><key app="EN" db-id="tzfezx99lprtaterppyvd2xyzdsvvrvedx2z" timestamp="1511171842">72</key></foreign-keys><ref-type name="Journal Article">17</ref-type><contributors><authors><author>Baba, T.</author><author>Ara, T.</author><author>Hasegawa, M.</author><author>Takai, Y.</author><author>Okumura, Y.</author><author>Baba, M.</author><author>Datsenko, K. A.</author><author>Tomita, M.</author><author>Wanner, B. L.</author><author>Mori, H.</author></authors></contributors><auth-address>Institute for Advanced Biosciences, Keio University, Tsuruoka City, Yamagata, Japan.</auth-address><titles><title>Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection</title><secondary-title>Mol Syst Biol</secondary-title></titles><periodical><full-title>Mol Syst Biol</full-title></periodical><pages>2006 0008</pages><volume>2</volume><edition>2006/06/02</edition><keywords><keyword>Escherichia coli/*genetics</keyword><keyword>Gene Deletion</keyword><keyword>Internet</keyword><keyword>Mutation</keyword><keyword>*Organisms, Genetically Modified</keyword></keywords><dates><year>2006</year></dates><isbn>1744-4292 (Electronic)
1744-4292 (Linking)</isbn><accession-num>16738554</accession-num><urls><related-urls><url>;(Baba et al., 2006)JW3359BW25113 ?mrcA::kan ADDIN EN.CITE <EndNote><Cite><Author>Baba</Author><Year>2006</Year><RecNum>72</RecNum><DisplayText>(Baba et al., 2006)</DisplayText><record><rec-number>72</rec-number><foreign-keys><key app="EN" db-id="tzfezx99lprtaterppyvd2xyzdsvvrvedx2z" timestamp="1511171842">72</key></foreign-keys><ref-type name="Journal Article">17</ref-type><contributors><authors><author>Baba, T.</author><author>Ara, T.</author><author>Hasegawa, M.</author><author>Takai, Y.</author><author>Okumura, Y.</author><author>Baba, M.</author><author>Datsenko, K. A.</author><author>Tomita, M.</author><author>Wanner, B. L.</author><author>Mori, H.</author></authors></contributors><auth-address>Institute for Advanced Biosciences, Keio University, Tsuruoka City, Yamagata, Japan.</auth-address><titles><title>Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection</title><secondary-title>Mol Syst Biol</secondary-title></titles><periodical><full-title>Mol Syst Biol</full-title></periodical><pages>2006 0008</pages><volume>2</volume><edition>2006/06/02</edition><keywords><keyword>Escherichia coli/*genetics</keyword><keyword>Gene Deletion</keyword><keyword>Internet</keyword><keyword>Mutation</keyword><keyword>*Organisms, Genetically Modified</keyword></keywords><dates><year>2006</year></dates><isbn>1744-4292 (Electronic)
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ADDIN EN.CITE.DATA (Potluri et al., 2010)pET28a-dacCpET28a-dacC28-400This workpGS100pGZ119EH derivative, contains TIR sequence downstream of?ptac, CamR ADDIN EN.CITE <EndNote><Cite><Author>Sperandeo</Author><Year>2006</Year><RecNum>5</RecNum><DisplayText>(Sperandeo et al., 2006)</DisplayText><record><rec-number>5</rec-number><foreign-keys><key app="EN" db-id="tzfezx99lprtaterppyvd2xyzdsvvrvedx2z" timestamp="1510914801">5</key><key app="ENWeb" db-id="">0</key></foreign-keys><ref-type name="Journal Article">17</ref-type><contributors><authors><author>Sperandeo, P.</author><author>Pozzi, C.</author><author>Deho, G.</author><author>Polissi, A.</author></authors></contributors><auth-address>Dipartimento di Scienze biomolecolari e Biotecnologie, Universita degli Studi di Milano, Milan, Italy.</auth-address><titles><title>Non-essential KDO biosynthesis and new essential cell envelope biogenesis genes in the Escherichia coli yrbG-yhbG locus</title><secondary-title>Res Microbiol</secondary-title></titles><periodical><full-title>Res Microbiol</full-title></periodical><pages>547-58</pages><volume>157</volume><number>6</number><edition>2006/06/13</edition><keywords><keyword>Aldose-Ketose Isomerases/biosynthesis/genetics</keyword><keyword>Arabinose/metabolism</keyword><keyword>Cloning, Molecular</keyword><keyword>Escherichia coli/genetics/metabolism/*physiology</keyword><keyword>Escherichia coli Proteins/*biosynthesis/genetics</keyword><keyword>Gene Expression Regulation, Bacterial</keyword><keyword>*Genes, Essential</keyword><keyword>Lipopolysaccharides/metabolism</keyword><keyword>Mutation</keyword><keyword>Open Reading Frames</keyword><keyword>Phosphoric Monoester Hydrolases/biosynthesis/genetics</keyword><keyword>Promoter Regions, Genetic</keyword><keyword>Sugar Acids/*metabolism</keyword></keywords><dates><year>2006</year><pub-dates><date>Jul-Aug</date></pub-dates></dates><isbn>0923-2508 (Print)
0923-2508 (Linking)</isbn><accession-num>16765569</accession-num><urls><related-urls><url>;(Sperandeo et al., 2006)pMN86pET21b-yebA40-440 ADDIN EN.CITE <EndNote><Cite><Author>Singh</Author><Year>2012</Year><RecNum>73</RecNum><DisplayText>(Singh et al., 2012)</DisplayText><record><rec-number>73</rec-number><foreign-keys><key app="EN" db-id="tzfezx99lprtaterppyvd2xyzdsvvrvedx2z" timestamp="1511172343">73</key></foreign-keys><ref-type name="Journal Article">17</ref-type><contributors><authors><author>Singh, S. K.</author><author>SaiSree, L.</author><author>Amrutha, R. N.</author><author>Reddy, M.</author></authors></contributors><auth-address>Centre for Cellular and Molecular Biology, Hyderabad 500007, India.</auth-address><titles><title>Three redundant murein endopeptidases catalyse an essential cleavage step in peptidoglycan synthesis of Escherichia coli K12</title><secondary-title>Mol Microbiol</secondary-title></titles><periodical><full-title>Mol Microbiol</full-title></periodical><pages>1036-51</pages><volume>86</volume><number>5</number><edition>2012/10/16</edition><keywords><keyword>Cysteine Endopeptidases/genetics/metabolism</keyword><keyword>Endopeptidases/genetics/*metabolism</keyword><keyword>Escherichia coli K12/*enzymology/genetics/growth & development/ultrastructure</keyword><keyword>Escherichia coli Proteins/genetics/metabolism</keyword><keyword>Microscopy, Electron, Scanning</keyword><keyword>Peptidoglycan/*metabolism</keyword></keywords><dates><year>2012</year><pub-dates><date>Dec</date></pub-dates></dates><isbn>1365-2958 (Electronic)
0950-382X (Linking)</isbn><accession-num>23062283</accession-num><urls><related-urls><url>;(Singh et al., 2012)pMUCαpJFK118EH derivative, for ectopic expression of PBP1BU. Bertsche, W. Vollmer, unpublishedpMUCα(mut)pJFK118EH derivative, for ectopic expression of PBP1B S510AU. Bertsche, W. Vollmer, unpublishedpMUC TG(mut)αpJFK118EH derivative, for ectopic expression of PBP1B E233QU. Bertsche, W. Vollmer, unpublishedpMUC TG(mut) α(mut)pJFK118EH derivative, for ectopic expression of PBP1B S510A E233QU. Bertsche, W. Vollmer, unpublishedpKD4oriR; AmpR KanR; source of kan cassetteDatsenko and Wanner, 2000pRS415pBR322 derivative; harbors the entire lac operon without promoter; AmpR ADDIN EN.CITE <EndNote><Cite><Author>Simons</Author><Year>1987</Year><RecNum>55</RecNum><DisplayText>(Simons et al., 1987)</DisplayText><record><rec-number>55</rec-number><foreign-keys><key app="EN" db-id="tzfezx99lprtaterppyvd2xyzdsvvrvedx2z" timestamp="1510916221">55</key></foreign-keys><ref-type name="Journal Article">17</ref-type><contributors><authors><author>Simons, R. W.</author><author>Houman, F.</author><author>Kleckner, N.</author></authors></contributors><titles><title>Improved single and multicopy lac-based cloning vectors for protein and operon fusions</title><secondary-title>Gene</secondary-title></titles><periodical><full-title>Gene</full-title></periodical><pages>85-96</pages><volume>53</volume><number>1</number><edition>1987/01/01</edition><keywords><keyword>Gene Expression Regulation</keyword><keyword>*Genetic Vectors</keyword><keyword>*Lac Operon</keyword><keyword>Operon</keyword><keyword>Protein Biosynthesis</keyword><keyword>Recombinant Fusion Proteins/*genetics</keyword><keyword>Recombinant Proteins/*genetics</keyword><keyword>Transcription, Genetic</keyword></keywords><dates><year>1987</year></dates><isbn>0378-1119 (Print)
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ADDIN EN.CITE.DATA (Hugonnet et al., 2016)pJEH12(ldtD)pACYC184 derivative; expresses LdtD under the IPTG-inducible trc promoter; TetR (Hugonnet et al., 2016)pAMS01(ldtE)pACYC184 derivative; expresses LdtE under the IPTG-inducible trc promoter; TetRThis workpAMS02(ldtF)pACYC184 derivative; expresses LdtF under the IPTG-inducible trc promoter; TetRThis workpSAV057ptrc99A derivative; contains weakened -35 promotor region (TTGACA-TTTACA); p15 origin; CamR ADDIN EN.CITE <EndNote><Cite><Author>Alexeeva</Author><Year>2010</Year><RecNum>126</RecNum><DisplayText>(Alexeeva et al., 2010)</DisplayText><record><rec-number>126</rec-number><foreign-keys><key app="EN" db-id="tzfezx99lprtaterppyvd2xyzdsvvrvedx2z" timestamp="1516788714">126</key></foreign-keys><ref-type name="Journal Article">17</ref-type><contributors><authors><author>Alexeeva, S.</author><author>Gadella, T. W., Jr.</author><author>Verheul, J.</author><author>Verhoeven, G. S.</author><author>den Blaauwen, T.</author></authors></contributors><auth-address>Molecular Cytology, Swammerdam Institute for Life Sciences, Faculty of Science, University of Amsterdam, Amsterdam, the Netherlands.</auth-address><titles><title>Direct interactions of early and late assembling division proteins in Escherichia coli cells resolved by FRET</title><secondary-title>Mol Microbiol</secondary-title></titles><periodical><full-title>Mol Microbiol</full-title></periodical><pages>384-98</pages><volume>77</volume><number>2</number><edition>2010/05/26</edition><keywords><keyword>Bacterial Proteins/metabolism</keyword><keyword>Carrier Proteins/metabolism</keyword><keyword>Cell Division</keyword><keyword>Cytoskeletal Proteins/metabolism</keyword><keyword>Escherichia coli/*metabolism</keyword><keyword>Escherichia coli Proteins/*metabolism</keyword><keyword>Fluorescence Resonance Energy Transfer</keyword><keyword>Membrane Proteins/metabolism</keyword><keyword>Penicillin-Binding Proteins/*metabolism</keyword><keyword>Peptidoglycan Glycosyltransferase/*metabolism</keyword><keyword>Serine-Type D-Ala-D-Ala Carboxypeptidase/*metabolism</keyword></keywords><dates><year>2010</year><pub-dates><date>Jul</date></pub-dates></dates><isbn>1365-2958 (Electronic)
0950-382X (Linking)</isbn><accession-num>20497333</accession-num><urls><related-urls><url>;(Alexeeva et al., 2010)pGS121pGZ119H derivative; expresses LdtE under the tac promoter; CamRThis workpGS123pGZ119H derivative; expresses LdtD under the tac promoter; CamRThis workpGS123C528ApGZ119H derivative; expresses LdtDC528A under the tac promoter; CamRThis workpGS124pGZ119H derivative, expresses LdtF under the tac promoter; CamRThis work ................
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