PDF Central Dogma of Genetics
[Pages:38]Central Dogma of Genetics
? Within each cell the genetic information flows from
? DNA to RNA to protein.
? This flow of information is unidirectional and irreversible. ? The information carried within the DNA dictates the end product
(protein) that will be synthesized.
? This information is the genetic code.
? Conversion of DNA encoded information to RNA
? is called transcription.
? The information from a mRNA is then translated to an amino acid sequence in the corresponding protein
1
Central Dogma
2 Molecular Biology of the Cell, 4th Edition.
How do we know RNA is intermediate and moves to Cytoplasm in Euks?
Grown briefly in Hot Uracil, spun out and grown in Cold Uracil.
Where does the radioactivity incorporated into RNA go?
3
RNA is Different than DNA
4
Transcription
? Process by which the genetic information is conveyed from a double stranded DNA molecule to a single stranded RNA molecule.
? Only one strand of DNA serves as a template:
? this is the transcribed or anti-sense strand.
? The complementary strand has a sequence identical to the RNA sequence (except for a U in place of a T),
? is called the sense strand, or the RNA-like strand.
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Designation of DNA strands
Sense strand Anti-sense strand
6
RNA is always 5' to 3'
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Salient Features of Transcription
? RNA polymerase:
? catalyzes the addition of one ribonucleotide at a time, ? extending the RNA strand being synthesized in the 5' to 3' direction.
? Promoter:
? DNA sequences near the beginning of a gene. ? These signal the RNA polymerase to begin transcription.
? Terminators:
? sequences within the RNA products, ? which signal the RNA polymerase to stop transcription.
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