BITC1311 Introduction to Biotechnology

Transformation of E. coli with a Recombinant Plasmid (adapted from Bio-Rad’s pGLO Transformation Kit) Objectives. Your performance will be satisfactory when you are able to: use sterile technique. make E. coli cells competent for transformation. transform E. coli with plasmid DNA. select for recombinant clones on antibiotic selection plates ................
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