The Inoue Method for Preparation and Transformation of ...
-80°C Frozen Heat Shock (HS) competent E. coli cells (400 L aliquots) Water bath or heat block (with water) at 42°C – check for this before you start! LB broth without selection and LB plates with appropriate selection. Collect an aliquot of HS competent E. coli from the -80°C freezer and thaw on ice. ................
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