The Inoue Method for Preparation and Transformation of ...
At its best, this method for preparing competent E. coli from Inoue et al. (1990) can challenge the efficiencies achieved by Hanahan (1983). However, under standard laboratory conditions, efficiencies of 1 x 108 to 3 x 108 transformed colonies/mg of plasmid DNA are more typical. ................
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