Calculating the diameter of high power based on your ...



MICROSCOPE CALCULATIONS47815504826000A) Microscope Magnification = Ocular Lens X Objective LensOcular lens: 10xLow power objective: 4xMedium power objective: 10xHigh power objective:40xWhat is the magnification under low power?What is the magnification under medium power?What is the magnification under high power?B) Measuring Low Power Field Diameter (field of view): the distance from one side to the other under each power when looking through the microscope.Low power: use a ruler to measure it. Use this distance for High & Medium field diameter calculations as follows.49911008255000Ex: low power field of view when measured with a ruler = 5 mm5154930292105 mm across005 mm acrossC) High & Medium field diameter must be calculatedThis formula is based upon the fact that the ratio of the diameters of the field of view is inversely proportional to the ratio of the magnifications.ieif the diameter doubles, the magnification is halvedieif the magnification doubles, the field diameter is halvedUsing the formula: Diameter of high power = magnification of low powerDiameter of low power magnification of high poweror DH = MLDL MHThe diameter under high power = magnification of low X diameter of lowmag highso,DH = ML x DL = 40x X 5 mm = 0.5 mmso, the field diameter of high power is 0.5 mmMH 400xFind the diameter of the field of view when using medium power. Dm = MLDL MMUsing the formula: Diameter of medium power = magnification of low powerDiameter of low power magnification of medium poweror DM = MLDL MMDM = ML x DL = 40x X 5 mm = 2 mmso, the field diameter of medium power is 2 mmMM 100xD)ACTUAL SIZE : Estimating the actual size of object in the field of view:Multiply the fraction that the object occupies in the field of view by the microscope field of view diameter.Ex: If 10 organisms could fit across the medium power field of view, the fraction of field of view occupied by the object is 1/10. 1714510795000506730102235actual size00actual sizeTo calculate its actual size we use the formula: Actual size = fraction of field of view occupied X field diameteror As = Ffo X FdActual size = 1/10 x 2 mm = 0.2 mmso, the actual size of the organism is 0.2 mmE)Drawing Magnification:You make an enlarged drawing of your organism. The drawing is obviously much bigger then the actual size of the organism (you need a microscope to see it.) How many times larger or smaller your drawing is compared to the actual size of the organism is the drawing magnification.ie: if you draw the organism 2 cm but its actual size is really only 1 mm, what is the magnification of the drawing?Use the formula magnification of drawing = drawing size or Dm = Ds = 2 cm__ = 20 mm = 100x actual size As 0.2 mm 0.2 mm3706495147955drawing00drawing9715514795500so, you drew the organism 100 times larger then it really is.MICROSCOPE _______________47815504826000A) Microscope Magnification = _______________ Lens X _______________LensOcular lens: 10xLow power objective: 4xMedium power objective: 10xHigh power objective:40xWhat is the magnification under low power?What is the magnification under medium power?What is the magnification under high power?B) Measuring ___________ Power Field Diameter (field of view): the distance from one side to the other under each power when ___________through the microscope.______power: use a ________to ___________it. Use this distance for ___________& ___________field diameter ___________as follows.49911008255000Ex: ________power field of view when measured with a ____________= 5 mm5154930292105 mm across005 mm acrossC) ____________& __________field diameter must be ____________This formula is based upon the fact that the ________of the diameters of the field of view is ___________proportional to the ratio of the magnifications.ieif the ____________doubles, the magnification is ______________ieif the ____________doubles, the field diameter is ___________Using the formula: Diameter of high power = magnification of low powerDiameter of low power magnification of high poweror DH = MLDL MHThe ______________under high power = magnification of low X diameter of lowmag highso,DH = ML x DL = 40x X 5 mm = 0.5 mmso, the field diameter of high power is 0.5 mmMH 400xFind the diameter of the ____________ of view when using _____________power. Dm = MLDL MMUsing the formula: Diameter of medium power = magnification of low powerDiameter of low power magnification of medium poweror DM = MLDL MMDM = ML x DL = 40x X 5 mm = 2 mmso, the __________ diameter of medium power is 2 mmMM 100xD)_______________ SIZE : Estimating the __________size of _______________in the field of ____________:Multiply the ____________that the object ______________in the field of view by the microscope field of view _________.Ex: If 10 organisms could ______across the ___________power field of view, the ____________of field of view occupied by the object is 1/10. 1714510795000506730102235actual size00actual sizeTo calculate its actual ________ we use the formula: Actual size = _________of field of view occupied X field ________or As = Ffo X Fd_________ size = 1/10 x 2 mm = 0.2 mmso, the actual size of the organism is _________E)__________Magnification:You make an ____________drawing of your ______________. The drawing is obviously much _____________then the actual size of the organism (you need a microscope to see it.) How many times _____________or smaller your ____________is compared to the actual _____________of the organism is the ____________magnification.ie: if you draw the organism ___________but its actual size is really only _________, what is the ______________of the drawing?Use the formula __________ of drawing = drawing _____ or ___ = Ds = ______ = 20 mm = _____x actual size As 0.2 mm 0.2 mmso, you drew the organism 20 times ____________ then it really is.211455139700003858895-502920drawing00drawing ................
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