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Table 1 SPREC 2.0, applied to fluid samples. Codes in bold come from the Laboratory Data Management System (LDMS). Type of sampleAscites fluidASCAmniotic fluidAMNBronchoalveolar lavageBALBlood (whole)BLDBone marrow aspirateBMABreast milkBMKBuccal cellsBUCUnficolled buffy coat, viableBUFUnficolled buffy coat, non-viableBFFFicoll mononuclear cells, viableCELFresh cells from non-blood specimen typeCENCells from non blood specimen type(e.g. ascites, amniotic), viableCLNCord bloodCRDCerebrospinal fluidCSFDried whole blood (e.g. Guthrie cards)DWBNasal washingNASFicoll mononuclear cells, non viablePELCells from non blood specimen type (e.g. ascites, amniotic), non-viablePENPleural fluidPFLPlasma, single spunPL1Plasma, double spunPL2Red blood cellsRBCSalivaSALSemenSEMSerumSERSputumSPTStoolSTLSynovial fluidSYNTearsTER24 h urineU24Urine, random (“spot”)URNUrine, first morningURMUrine, timedURTOtherZZZType of primary containerAcid citrate dextrose ACDAdditivesADDSerum tube without clot activatorCATCitrate phosphate dextroseCPDCell Preparation Tube?CPTEDTA and gelEDGLithium heparinHEPHirudinHIRLithium heparin and gelLHGOragene collection container or equivalentORGPAXgene? blood RNA+PAXPotassium EDTAPEDPolyethylene tube sterilePETS8820 protease inhibitor tablets or equivalentPI1Protease inhibitorsPIXPolypropylene tube sterile PPSPAXgene? blood DNAPXDPAXgene? bone marrow RNAPXRSodium citrateSCISodium EDTASEDSodium heparinSHPSodium fluoride/potassium oxalateSPOSerum separator tube with clot activatorSSTTempus? tubeTEMTrace elements tubeTRCUnknownXXXOtherZZZPre-centrifugation (delay between collection and processing)RT*<2 hA2 to 10 °C<2 hBRT2-4 hC2 to 10 °C2-4 hDRT4-8 hE2 to 10 °C4-8 hFRT8-12 hG2 to 10 °C8-12 hHRT12-24 hI2 to 10 °C12-24 hJRT24-48 hK2 to 10 °C24-48 hLRT>48 hM2 to 10 °C>48 hN35 to 38 °C<2 hOUnknownXOtherZCentrifugationRT 10 to 15 min<3000 g no brakingART 10 to 15 min<3000 g with brakingB2 to 10 °C 10 to 15 min<3000 g no brakingC2 to 10 °C 10 to 15 min<3000 g with brakingDRT 10 to 15 min3000 g to 6000 g with brakingE2 to 10 °C 10 to 15 min3000 g to 6000 g with brakingFRT 10 to 15 min6000 g to 10000 g with brakingG2 to 10 °C 10 to 15 min6000 g to 10000 g with brakingHRT 10 to 15 min>10000 g with brakingI2 to 10 °C 10 to 15 min>10000 g with brakingJRT 30 min<1000 g no brakingMNo centrifugationNUnknownXOtherZSecond centrifugationRT 10 to 15 min<3000 g no brakingART 10 to 15 min<3000 g with brakingB2 to 10 °C 10 to 15 min<3000 g no brakingC2 to 10 °C 10 to 15 min<3000 g with brakingDRT 10 to 15 min3000 g to 6000 g with brakingE2 to 10 °C 10 to 15 min3000 g to 6000 g with brakingFRT 10 to 15 min6000g to 10000g with brakingG2 to 10 °C 10 to 15 min6000 g to 10000 g with brakingHRT 10 to 15 min>10000 g with brakingI2 to 10 °C 10 to 15 min>10000 g with brakingJNo centrifugationNUnknownXOtherZPost-centrifugation delay<1 h 2 to 10 °CA<1 h RTB1 to 2 h 2 to 10 °CC1 to 2 h RTD2 to 8 h 2 to 10 °CE2 to 8 h RTF8 to 24 h 2 to 10 °CG8 to 24 h RTH>24 h 2 to 10 °CI>24 h RTJNot applicableNUnknownXOtherZLong-term storagePP tube 0.5- to 2-mL**(-85) to (-60) ° CAPP tube 0.5- to 2-mL(-35) to (-18) °CBPP tube 0.5- to 2-mL<-135 ° CVCryotube 1- to 2-mLLN***CCryotube 1- to 2-mL(-85) to (-60) °CDCryotube 1- to 2-mLProgrammable freezing to <-135 °CEPlastic cryo strawLN***FStraw(-85) to (-60) °CGStraw(-35) to (-18) °CH StrawProgrammable freezing to <-135 °CIPP tube ≥ 5 mL(-85) to (-60) °CJPP tube ≥ 5 mL(-35) to (-18) °CKMicroplate(-85) to (-60) °CLMicroplate(-35) to (-18) °CMCryotube 1- to 2-mLLN*** after temporary (-85) to (-60) °CNPlastic cryo strawLN*** after temporary (-85) to (-60) °COParaffin blockRT or 2 to 10 °CPBagLN***QDry technology mediumRTRPP tube 40- to 500-?L(-85) to (-60) °CSPP tube 40- to 500-?L(-35) to (-18) °CTPP tube 40- to 500-?L<-135 ° CWOriginal primary container(-35) to (-18) °C or (-85) to (-60) °CYUnknownXOtherZ*RT, room temperature: 18 to 28 °C**PP, polypropylene***LN, liquid nitrogen, referring to either vapor- or liquid-phase (this information being documented in the biobank’s SOPs)Volumes refer to container sizeTable 2 SPREC 2.0, applied to solid samples. Codes in bold come from the Laboratory Data Management System (LDMS). Type of sampleFresh cells from non blood specimen type (e.g. biopsy)CENCells from non blood specimen type (e.g. dissociated tissue), viableCLNCells from fine needle aspirateFNAHairHARCells from laser capture microdissected tissue LCMCells from non blood specimen type (e.g. dissociated tissue), non viablePENPlacentaPLCSolid tissueTISDisrupted tissue, non-viableTCMOtherZZZType of collectionAutopsy < 6 h post-mortemA06Autopsy 6-12 h post-mortemA12Autopsy 12-24 h post-mortemA24Autopsy 24-48 h post-mortemA48Autopsy 48-72 h post-mortemA72Biopsy in culture mediaBCMBiopsyBPSBiopsy in normal saline or phosphate buffered salineBSLBiopsy in tissue low temperature transport mediaBTMFine needle aspirateFNAPunctionPUNSurgical excision in culture mediaSCMSurgical excisionSRGSurgical excision in normal saline or phosphate buffered salineSSLSurgical excision in tissue low temperature transport mediaSTMSurgical excision in vacuum containerVACSwabSWBOtherZZZWarm ischemia time< 2 minA2 to 10 minB10 to 20 minC20 to 30 minD30 to 60 minE>60 minFUnknownXNot applicable (e.g. biopsy)NOtherZCold ischemia time< 2 minA2 to 10 minB10 to 20 minC20 to 30 minD30 to 60 minE>60 minFUnknownXNot applicable (e.g. autopsy)NOtherZFixation / stabilization typeNon-aldehyde with acetic acidACAAldehyde-basedALDAllprotect ? tissue reagentALLAlcohol-based ETHNon-buffered formalinFORHeat stabilizationHSTSnap freezingSNPNon-aldehyde based without acetic acidNAANeutral buffered formalinNBFOptimum cutting temperature mediumOCTPAXgene? tissuePXTRNA Later?RNLUnknownXXXOtherZZZFixation time<15 minA15 min to 1 hB1 to 4 hC4 to 8 hD8 to 24 hE24 to 48 hF48 to 72 hGNot applicableNUnknownXOtherZLong-term storagePP tube 0.5- to 2-mL**(-85) to (-60) °CAPP tube 0.5- to 2-mL(-35) to (-18) °CBPP tube 0.5- to 2-mL<-135 ° CVCryotube 1- to 2-mLLiquid nitrogen***CCryotube 1- to 2-mL(-85) to (-60) °CDCryotube 1- to 2-mLProgrammable freezing to <-135 °CEPlastic cryostrawLiquid nitrogenFStraw(-85) to (-60) °CGStraw(-35) to (-18) °CH StrawProgrammable freezing to <-135 °CIPP tube ≥ 5 mL(-85) to (-60) °CJPP tube ≥ 5 mL(-35) to (-18) °CKMicroplate(-85) to (-60) °CLMicroplate(-35) to (-18) °CMCryotube 1- to 2-mLLN*** after temporary (-85) to (-60) °CNStrawLN*** after temporary (-85) to (-60) °COParaffin blockRT or 2 to 10°CPBagLN***QDry technology mediumRTRPP tube 40 to 500?L(-85) to (-60) °CSPP tube 40 to 500?L(-35) to (-18) °CTPP tube 40- to 500-?L<-135 ° CWOriginal primary container(-35)-(-18) °C or (-85)-(-60) °CYUnknownXOtherZ*RT, room temperature: 18 to 28 °C**PP, polypropylene***Liquid nitrogen refers to either vapor or liquid phase (this information being documented in the biobank’s SOPs)Volumes refer to container size ................
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