PCR
c. Extend using DNA polymerase (Taq @ 72 °C) Time depends on length and concentration of template. Repeat cycle up to ~ 30 times. Limited by stability of polymerase, concentration of primers and dNTPs C. Analysis. 1. Gel electrophoresis (what we do) a. Gel purification--cut out bands and reisolate product using Qiagen kit. b. ................
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