DNA Replication, Transcription, Translation, and Mutation ...



DNA Replication, Transcription, Translation, and Mutation Worksheet

[pic]Cystic Fibrosis Gene:  When both copies of this gene are mutant, individuals have lung and kidney problems.

You will be able to write DNA sequence for the complementary strand (1).

You will be able to write the RNA and protein sequence (2 & 3).

You will be able to note the consequence of each DNA mutation for the protein.

A. Normal Gene (a small portion only)

5'- acc att aaa gaa aat atc atc ttt ggt gtt tcc tat gat - 3'

1.

2.

3.

B. Known Mutant Gene

5' - acc att aaa gaa aat atc atc --- ggt gtt tcc tat gat - 3'

1.

2.

3.

C. Hypothetical Mutant Gene 1

5' - acc att aaa gaa aaa atc atc ttt ggt gtt tcc tat gat- 3'

1.

2.

3.

D. Hypothetical Mutant Gene 2

5' - acc att aaa gaa caa tat cat ctt tgg tgt ttc cta tga t - 3'

1.

2.

3.

Mutation - a change in the DNA

Point mutations - when a single nitrogen base is deleted, inserted or substituted.

Frameshift Mutation- when many amino acids coded for are different because of an  insertion or deletion.

The following example shows how these 3 kinds of mutations work.  Fill out the RNA and protein for each and compare the mutations to the original.  The mutation is in bold.

 

Original DNA   T A C  G C G  T G C  A C G  A T G  C A G  T A G  T A C

  RNA

  Protein

Mutation 1 DNA   T A C  G C G  T G C  A C G  A T C  C A G  T A G  T A C

  RNA

  Protein

 

Type of Mutation:

Mutation 2 DNA   T A C  G C G  T G C  T C G  A T G  C A G  T A G  T A C

  RNA

  Protein

 

Type of Mutation:

Mutation 3 DNA   T A C  G C G  C T G  C A C  G A T  G C A  G T A  G T A  C

  RNA

  Protein

 

Type of Mutation: 

 

 

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