Preparing SDS-PAGE gels
"Helper" = E. coli strain carrying pRK2013 or some other helper plasmid that will promote mobilization of broad host range plasmid. pRK2013 will not replicate in Agrobacterium and many other recipients. "Recipient" = Agrobacterium or Rhizobium, often grown on LB at 28oC, or . ................
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