Dmg5c1valy4me.cloudfront.net



Shamayeeta Ray, Ph. D.

Postdoctoral Fellow, Department of Molecular and Cellular Biology,

Harvard University, 52 Oxford Street, Cambridge, MA 02138, USA

Contact No.- +1-5712531101(cell)

Email: shamayeeta.ray@, shamayeeta_ray@fas.harvard.edu

Date of Birth: 12/03/1987 (mm/dd/yy)

Academic Records

2012- 2017: Ph.D.

IITB-Monash Research Academy (collaboration PhD between Indian Institute of

Technology Bombay and Monash University Australia)

Supervisor: Prof. Ruchi Anand, Dr. Santosh Panjikar

2009-2011: M. Sc. Biotechnology

University of Calcutta

Marks obtained: 73%, CGPA: 4.59/6

2006-2009: B. Sc. Chemistry;

St. Xavier's College, University of Calcutta

Marks obtained: 76.6%, CGPA: 7.88/10

2004-2006: Higher Secondary Examination (10+2);

West Bengal Council of Higher Secondary Education

Marks Obtained: 88.8%

2002-2004: Secondary Examination (10);

West Bengal Board of Secondary Education

Marks Obtained: 89.8%

Professional Experience (projects undertaken)

April 2018- present - Postdoctoral Research (Harvard University, USA)

Supervisor: Prof. Rachelle Gaudet, Department of Molecular and Cellular Biology, Harvard University, MA, USA

Project title: “ Molecular mechanism of metal transport by NRAMP family transporters”

July 2012 - November 2017 - Ph. D (IIT Bombay, India and Monash University, Australia)

Supervisor: Dr. Ruchi Anand, Department of Chemistry, IIT Bombay

Dr. Santosh Panjikar, Department of Biochemistry, Monash University, Australia

Project title: “Structural characterization of regulatory proteins useful in developing biosensors for water purification system”

January 2011 - June 2011 – Master’s Dissertation (University of Calcutta, India)

Supervisor: Dr. Anindita Seal, Department of Biotechnology, University of Calcutta, Kolkata

Project title: “Full Length cloning and functional study of substrate binding site of BjYSL8.1 using domain swap”

May 2010 - July 2010 – Summer project (Indian Institute of Science, Bangalore, India)

Supervisor: Dr. Raghavan Varadarajan, Molecular Biophysics Unit, IISc, Bangalore

Project title: “Effect of single point mutation at exposed site of CcdB on the protein structure and folding”

Publications

[1] Ray, S., Gunzburg, M. J., Wilce, M., Panjikar, S., and Anand, R. (2016) Structural Basis of Selective Aromatic Pollutant Sensing by the Effector Binding Domain of MopR, an NtrC Family Transcriptional Regulator, ACS Chem. Biol. 11(8), 2357–2365.

[2] Ray, S., Panjikar, S., and Anand, R. (2017) Structure Guided Design of Protein Biosensors for Phenolic Pollutants, ACS Sens. 2(3), 411-418.

[3] Ray, S., Maitra, A., Biswas, A., Panjikar, S., Mondal, J., and Anand, R. (2017) Functional Insights into the Mode of DNA and Ligand Binding of the TetR Family Regulator TylP from Streptomyces fradiae, J. Biol. Chem. 292, 15301-15311.

[4] Ray, S., Singh, J., Panjikar, S., and Anand, R. Structural Insights into the Mode of Signal Transduction and Allostery in Phenolic Pollutant Binding Regulators (Manuscript under preparation).

[5] Ray, S., Senapati, T., Sahu, S., Bandyopadhyaya, R., and Anand, R. Design of Ultrasensitive Protein Biosensor Strips for Selective Detection of Aromatic Contaminants in Environmental Wastewater (Manuscript under revision in Analytical Chemistry).

[6] Ray, S., Panjikar, S. and Anand, R. Design of Protein based Biosensors for Selective Detection of Benzene Group of Pollutants (Manuscript under revision in ACS Sensors).

Patents

[1] Anand, R. and Ray, S. (2018) Biosensors for detecting organic pollutants and Process for producing the same, IN Patent, 201821003722.

[2] Anand, R., Ray, S., Senapati, T., and Bandyopadhyaya, R. (2018) Ultrasensitive protein biosensor strips for selective detection of aromatic contaminants and method of preparation thereof, IN Patent, 201821016035.

Conference proceedings

[1] Ray, S., Panjikar S, Anand R. (2016) Structural Basis of Selective Aromatic Pollutant Sensing by MopR, an NtrC Family Transcriptional Regulator, Acta Cryst., A72, s249.

[2] Ray, S., Panjikar S, Anand R. (2017) Structure guided design of aromatic biosensors for water quality monitoring, Found. Cryst., 70, C97.

Symposium and conferences

2017: Oral presentation in International Union of Crystallography (IUCr) -2017, Hyderabad

2017: Oral presentation in Biophysics Paschim Symposium-2017, TIFR, Bombay

2016: Oral presentation in National Symposium of Crystallography (NSC)-2016, IISER, Pune.

2016: Oral presentation in In House (Chemistry) Symposium (IHS)-2016, IIT Bombay

2016: Poster presentation in European Crystallography Meeting (ECM)-2016, Switzerland

2016: Poster presentation in WRCB Industry Day-2016, IIT Bombay

2016: Participated in Advances in Glyco-chemistry Symposium, IIT Bombay

2016: Poster presentation in Workshop on Clean Water Technology -2016, IIT Bombay

2015: Poster presentation in Asian Crystallography Association Symposium (ASCA), Kolkata

2015: Poster presentation in Western Frontiers Structural Meet -2015, IIT Bombay

2015: Oral presentation in Symposium of IITB-Monash Research Academy, IIT Bombay

2014: Oral presentation in Symposium of IITB-Monash Research Academy, IIT Bombay

Awards and Honours

1. Recipient of IIT Bombay International Travel Support, based on research performances.

2. Recipient of Best Poster Award in Clean Water Technology Workshop (2016) at IIT Bombay

3. Recipient of Prime Minister's Fellowship ( for 4 years) from DST, Govt. of India for

pursing Ph. D in IIT Bombay

4. Recipient of Summer Research Fellowship from the Indian Academy of Science (IAS) for

pursuing summer project in IISc, Bangalore.

5. Inspire Scholarship, Department of Science and Technology (Top 1% in 12th Standard

Board Examination)

6. Was among top 50 from the state (Top 0.5% ) in 10th Standard Board Examination

7. Received a GRE score of 314/340 and a TOEFL score of 108/120

Teaching Experience

2013: Teaching Assistant for Advanced Lab techniques (Physical Chemistry), Chemistry Department, IIT Bombay, India

2014-15: Research mentor for SK Sahoo (for his Msc Thesis), Chemistry Department, IIT Bombay, India

2015: Research mentor for Anjum Sheikh (Project Assistant), Chemistry Department, IIT Bombay, India

2015-16: Research mentor for Pranay Joshi (for his Msc Thesis), Chemistry Department, IIT Bombay, India

2016: Research mentor for Sreelaja Sasi (Project Assistant), Chemistry Department, IIT Bombay, India

2016-17: Research mentor for Anwesha Maitra (for her Msc Thesis), Chemistry Department, IIT Bombay, India

2016-17: Research mentor for Piyush Goyal (for his Msc Thesis), Chemistry Department, IIT Bombay, India

2017-18: Research mentor for Tamasri Senapati (for her Msc Thesis), Chemistry Department, IIT Bombay, India

2018: Research mentor for Agnele Sewa (Summer Intern, SROH), Molecular and Cellular Biology Department, Harvard University, Cambridge, MA

Proficiency and Skills

• Molecular Biology: Genomic DNA isolation; Competent cell preparation; Cloning in expression and luciferase vectors; Site-directed mutagenesis (kit based and overlap PCR based); Synthesis and purification of short DNA (using PAGE and column method)

• Proteomics: Expression and purification of soluble and membrane proteins by affinity and size-exclusion chromatography; In vitro (colorimetric) activity assay of ATPase proteins; EMSA (DNA-protein interaction study)

• Structural Biology: Crystallization of soluble and membrane proteins and optimization of crystals (using micro seeding and streak seeding); crystal mounting, screening and data collection (in synchrotron and home source); data processing; structure solution; structure refinement.

• Biophysical Chemistry: Fluorescence (steady state and time resolved); CD; Thermal shift assay; Gel Filtration; ITC; SPR; MALS; Luciferase based chemi-luminescence assay

• Bioinformatics: Sequence alignment using ClustalW, ESPript; Primer designing using IDT oligoanalyzer, Primer express; Sequence similarity network using Cytoscape; Docking using Autodock

• Softwares: Microsoft Power Point, Adobe Illustrator, Pymol, Coot, CCP4, PHENIX, HKL2000, Origin, ChemDraw, Graphpad Prism

................
................

In order to avoid copyright disputes, this page is only a partial summary.

Google Online Preview   Download