Lab
Lab.13 Sporozoa
Family : plasmodiidae
Genus : Plasmodium ( causing the malaria )
P.malariae , P.vivax , P.falciparum , P.ovale ( in human )
P.gallinaceum , P.relictum ( in birds )
Definitive Hosts: Rodents, Rabbits, Birds, and human
Final host : Mosquites ( Anopheles , Culex )
Infective Site: Blood ( RBCs ) , Liver
Infective Stage: sporozoite ( Sporozoites are motile and they move by gliding, centrally located nucleus )
* Ring form : the cytoplasmic ring in the RBCs . The parasite may be first visible as a crescent – shaped mass at the outer edge of the red blood cell .
* Developing trophozoites : the parasite takes on more of an irregularly shaped ameboid appearance .
* Schizont : characterized by the presence of multiple chromatin bodies , The merozoites (non-motile) surrounded by cytoplasmic material occupy the majority of the RBCs .
* Gametocytes : A male gametocyte growing to give many flagellated ( micro -
gametocytes ) , whereas the female gametocyte differentiates to macrogametocytes .
* Diagnosis : - clinical signs are associated with anemia and fever.
- Thick and thin blood smears staind with giemsa stain and Serology diagnosis .
[pic]
[pic]
[pic]
Family : Theileriidae
Genus : Theileria
T. parva : The cause of bovine Theileriosis and East coast fever .
T. annulata : Also the cause of bovine and T. equi Causing Equine prioplasmosis Definitive Hosts: Cattle, Sheep/Goats .
Final host : Ticks
Infective Stage: sporozoite .
Infective Site: Blood (lymphocytes "WBCs", and erythrocytes "RBCs ")
* There are two pictures for schizont (Kochs blue bodies) macroschizonts and microschizonts that produce the micromerozoites in the red blood cell.
Diagnosis :
1- Clinical Signs (Diarrhea, loses weight rapidly, anemia and fever.
2- Blood smears with Giemsa stain and In Ramonowsky-stained smears, schizonts appear as bluish bodies, approximately 8-12 microns in diameter with multiple reddish nuclei .Intraerythrocytic piroplasms are similar to small babesial parasites. They are approximately 0.5-2.5 microns and pleomorphic, but most often appear rod-shaped, round or oval.
3- Serological test (PCR and ELISA).
[pic]
Schizont in RBC Merozoite
[pic]
Family : Babesiidae
Genus : Babesia
Babesia is pear , roundish and irregular shape in RBCs, have two form ( large and small ) that causes a hemolytic disease known as Babesiosis & Texas cattle fever . can be transmitted from human to human either through the tick vector or through blood transfusions .
Definitive Hosts: Cattle, Sheep/Goats , Horse and dog .
Babesia microti infected the human , life cycle involves two hosts ( tick and mouse )
Final host : Hard Ticks ( Ixodidae )
Infective Stage: sporozoite .
Infective Site: Blood .
Diagnosis :
1- clinical symptoms: such as high fever, anemia, and hemoglobinuria, in the infected hosts.
2- Thick and Thin blood sample testing : the cell contain vacuoles, and the lack of pigment production. Trophozoites morph into merozoites that appear in a tetrad formation( Maltese-cross form) .
3- Serodiagnosis indirect fluorescent antibody (IFA) test .
[pic]
Babesia canis
Trophozoites
[pic]
[pic]
................
................
In order to avoid copyright disputes, this page is only a partial summary.
To fulfill the demand for quickly locating and searching documents.
It is intelligent file search solution for home and business.
Related searches
- summa lab 95 arch street
- abstract for chemistry lab report
- quest diagnostics lab locations
- ap biology photosynthesis lab answers
- et50 photosynthesis lab ap bio
- ecological succession lab answer key
- lab urine test detection times
- ap bio photosynthesis lab answers
- photosynthesis lab ap biology
- elodea photosynthesis lab answers
- elodea photosynthesis lab results
- photosynthesis lab report experiment