Test Review on DNA Structure, DNA Replication
Study Guide for DNA Structure, DNA Replication
and Gene Expression (Transcription and Translation): Chapter 8
Be able to explain the structure and function of DNA.
• Understand that DNA is a double helix composed of two strands of nucleotides and be able to describe this shape.
• Know the three parts of a nucleotide.
• Understand that the nitrogen base is the part of the nucleotide that forms the genetic code, and be able to name the four possible nitrogen bases in a DNA nucleotide.
• Know how the nucleotides are organized in the strands
• Know the complementary nitrogen base pairings
Be able to explain the overall process of DNA replication including:
• The roles of the enzymes helicase and DNA polymerase
• Origin of each strand in the daughter DNA molecules
• When and where the process occurs
Understand that each gene is the information to build one protein or polypeptide chain in a protein.
Be able to explain that gene expression (producing the protein encoded by a gene) involves two steps, transcription and translation.
Transcription:
• Be able to explain the purpose of transcription
• Be able to compare and contrast DNA and RNA (at least 3 differences)
• Be able to explain the process of transcription including the role of RNA polymerase
• Be able to explain the processing of eukaryotic mRNA (removal of introns)
Translation
• Be able to explain the process of translation including:
▪ The purpose of translation
▪ The site of translation
▪ Structure of a tRNA molecule. Be able to compare and contrast the structure and function of mRNA molecules and tRNA molecules.
▪ Be able to explain what a codon is in mRNA and an anticodon in tRNA
▪ Be able to interpret a genetic code chart to determine what amino acid sequence will be produced from a given mRNA sequence
▪ Understand that the genetic code is universal in all organisms and know the significance of this for biotechnology.
Be able to define mutation and mutagen. Know the difference between a point mutation (missense mutation) and a frameshift mutation (nonsense mutation).
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