Johns Hopkins Medicine, based in Baltimore, Maryland



A. ??Tissue Culture Protocols:Dissociated Primary Cortical Neuron CultureEmbryonic day 18 rats?Dissociated via TrypsinEmbryonic day 18 or Post-natal day 1-2 miceDissociated via Papain?Embryonic day 18 single mouseDissociated via Trypsin?Dissociated Primary Hypothalamic Neuron CultureEmbryonic day 16-17 ratsDissociated via PapainDissociated Primary Hippocampal Neuron CultureEmbryonic day 18 ratsPrimary Astrocyte Cell CulturePostnatal day 1-3 ratsDissociated Primary Olfactory Receptor Neuron CulturePostnatal day 1-2 ratsPostnatal day 1-2 mouseAdult human tissueDissociated Primary Olfactory Bulb Neuron CultureEmbryonic day 18 rat & mice, Postnatal day 1-2 rat & miceOlfactory Bulb and/or Olfactory Epithelial Explant CulturesEmbryonic day 16 miceCell Lines3T3-L1APC-12B.??Biochemical Assay Protocols:Fatty Acid Oxidation of 14C-PalmitateAcetate Incorporation Assay - Fatty Acid Synthase Activity AssayMeasurement of Intracellular ATPAMPK/pAMPK Western Blot AnalysisAMPK Activity Assay Protocol - SAMS Peptide AssayCellular Viability AssaysCalcein and Propidium Iodide AssayXTTAdenylyl Cyclase Assay (with Alpha Toxin)Guanylyl Cyclase Assay?C.??Immunostaining Protocols:General ImmunohistochemistryGFAP, NST ImmunohistochemistryBRDU, NST ImmunocytochemistryDAPI StainingTunel StainingWhole Mount Staining ProtocolsFluorescent StainingX-GAL Staining for Whole MountsOther Whole Mount X-Gal ProtocolsOE1, GAP43 Fab ImmunohistochemistryLectin StainingNT-3 with amplificationD.??Molecular Biology Protocols:Real-Time (RT) PCRChromatin Immunoprecipitation (ChIP)In Situ Hybridization ................
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