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Transciption: DNA mRNA WebquestIntroduction: The first step of protein synthesis is known as transcription. During transcription, DNA is made into a single strand mRNA in the nucleus and then gets transported to the cytoplasm. In today’s webquest you will see how the process of transcription proceeds.Go to iLearn and click on “Transcription Overview”. Click launch activity and then work your way through and stop at translation.Overview_________________________ are built inside cells using the _____________code as an instruction manual for how to _________________ each protein. DNA is located in the _________________________ of every cell and stored in the form of structures called ______________________. An organism may have just a few or ___________ chromosomes.DNA is __________________ up tightly to form a chromosome. Chromosomes are large enough to be _________________ using a light microscope, but seeing a single strand of DNA requires ______________________________________ microscopes.Looking more closely at a segment of a single chromosome, the DNA’s ______________________ can be seen.The entire strand of DNA is too _________________ to travel outside of the nucleus to the ______________________, where the _________________________ for building proteins are located.Therefore, the DNA code of the single _________ encoding the protein being produced is read by an __________________ and an ____________ copy is made.The first step in protein production is called __________________________.The mRNA is small enough to travel ___________________ the nucleus to the ____________________.The second step of protein production is a process called ______________________________.The code of the mRNA is read by an organelle, which _____________________ the code and links ____________________ together in the proper order to form a ______________________.Once the amino acid chain is complete, it folds up into a __________________________ structure. This is now a functional protein.The protein then travels to where it is needed to perform it’s _____________________ in the cell.TranscriptionThe process of protein synthesis takes place in _________________ steps. During the first step, called ______________________, an mRNA copy of the gene is made which carries the encoded information to the __________________________.Every gene has ______________ regions that occurring the following order. The first is the ______________________, which turns the gene on and off.The second region is the _____________________ region, which encodes the information for which protein to ___________________________.The third region is the _____________________ sequence, which signals the end of the gene.______________________________ molecules are present in the nucleus. This ____________ is responsible for reading the DNA code and building the mRNA strand.The promoter region of the gene acts as a ______________________. It signals when to turn the gene on and off.The RNA polymerase molecule recognizes that the _________________ is signaling to produce the protein.The RNA polymerase molecule binds to the ____________________ and the DNA double helix ________________________.Once the RNA polymerase reaches the _____________________, it reads a _______________ strand. As it does this, it builds the mRNA copy.As RNA polymerase reads each ________________, it brings in the complementary nucleotide and bonds them together forming the mRNA strand. The nucleotides break ____________ from the DNA, and the DNA folds back into a _______________________________.When RNA polymerase reaches the ______________________ sequence, the sequence causes the mRNA to fold back on itself.This prevents transcription from _______________________ and both the RNA polymerase and mRNA strand fall off the DNA strand.The mRNA strand then exits the nucleus and enters the cytoplasm.Go to iLearn and click on “Drag and Drop Protein Synthesis” then click on “Transcription”In transcription Adenine bonds with Uracil and Guanine bonds with Cytosine. Fill out the table below.Which base in RNA is replaced by uracil?What is the name of the enzyme that creates the mRNA copy from DNA?What is the name of the sugar in the mRNA nucleotides?What is the mRNA transcript for the DNA sequence T T A C G C ?Go to iLearn and click on “Transcribe DNA Sequence”Transcribe the following DNA sequence as you did on the computer:TopBot A T T A C G A T C T G C A C A A G A T C C TTranscribe the following DNA sequence using what you learned today in the webquest:Top T A G G A T G A C T A C T A G C T A C G A T T C G T C Bot ................
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