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(GenBank accession KP851975.1) as a template. PCR thermocycler settings for vapN amplification consisted of an initial 5 min at 95°C and 30 cycles of 95°C for 30 s, 60°C for 30 s, and 72°C for 2 min followed by 10 min at 72°C.R. equi reference strains 33707 and 6939 (ATCC©, USA) were used as controls for the virulent and avirulent plasmid phenotypes, respectively. ................
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