Original article UDC: 616.348-006.6:577
Original article
UDC: 616.348-006.6:577.11 doi:10.5633/amm.2016.0305
PARAMETERS OF OXIDATIVE STRESS IN COLON CANCER TISSUE
Andrej Veljkovi1, Goran Stanojevi1,2, Branko Brankovi1,2, Dusica Pavlovi1, Ivana Stojanovi1, Tatjana Cvetkovi1,2, Tatjana Jevtovi1, Dusan Sokolovi1, Jelena Basi1, Milena Despotovi1, Branka orevi1, Gordana Koci1
Colorectal cancer is one of the most frequent neoplastic diseases in the human population, and one of the most frequent causes of death. Reactive oxygen species (ROS) are involved in the process of cancer initiation and progression. It is known that ROS are formed in excess in chronic diseases of the gastrointestinal tract, but the precise mechanisms of oxidative stress being induced in cancer cells and the role of ROS in colorectal cancer progression are still not exactly understood.
Tumor tissue specimens as well the healthy colon tissue and the tissue surrounding the tumor were obtained from 50 primary colorectal cancers. The concentration of TBARS in the homogenate was determined by spectrophotometric method by Andreeva et al. AOPP concentrations in the tissue was measured by the spectrophotometric method by Vitko et al. Catalase activity in plasma was determined by spectrophotometric method by Goth.
TBARS and AOPP levels were significantly higher in the tumor tissue compared to the control healthy tissue (p ................
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