UDC 577.2:57.084.1:616—097 Biosynthesis of the scFv Antibody to Human ...

E. coli XL1-Blue. The gene structure in the resulting pET22F11barstar was verified by sequenc-ing, which followed Sanger’s method [13]. To obtain the recombinant protein, E. coli BL21(DE3) was transformed with pET22F11barstar, and transformants grown in liquid LB. At the log phase, the culture was supplemented with 0.1 mM IPTG and incubated ... ................
................