Cell
Supplemental data file 4Pathology Report on Infant B from SCNT of Cumulus Cell Female cynomolgus monkey (Infant B) was born by caesarean section on day 137 of pregnancy. The newborn monkey was found dead approximately 30 hours after birth and was preserved in a freezer (-80 ℃) until a gross examination on 2018-01-12. The cause of death for this animal was most likely to be the neonatal respiratory distress syndrome (RDS).Macroscopically, multifocal dark red discoloration was noted in the right cranial and caudal lungs lobes, collapse or incomplete expansion was noted in most of the lung lobes (Figure 1 below). Microscopically, characteristic changes in the lungs included variably collapsed or fluid-filled alveoli; the alveoli are lined by round epithelial cells, alveolar septal congestion, and the presence of acidophilic hyaline membranes that line terminal bronchioles, alveolar ducts and alveoli (Figure 2 below). The presence of extensive neonatal atelectasis is typically a feature of neonatal respiratory distress syndrome. These factors can contribute to RDS due to the deficiency of pulmonary surfactant, decreased arterial blood flow, and amniotic fluid aspiration.Figure 1: Representative gross picture of the lungs.Figure 2: Representative microphoto of the lungs.Report by Tiansheng Zhou, MSc, certificated pathologist of Chinese College of Veterinary Pathologist & Chinese Society of ToxicologyInformation on Infant A from SCNT of Cumulus CellFemale cynomolgus monkey (Infant A) was born by caesarean section on day 135 of pregnancy. The newborn monkey was found dead approximately 3 hours after birth and was preserved in a freezer (-80 ℃). No pathology report was requested, because the monkey showed grossly retarded body development, with disproportionally small body trunk. The animal care person noted breathing difficulty of the monkey prior to the death. ................
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