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CURRICULUM VITAEPERSONAL INFORMATIONS Name: Rahul KrishnanDate of birth: 17-12-1991Sex: MaleAddress:AswathyCheriyanadu P.O Chengannur, AlappuzhaKerala, IndiaPIN. 689511Ph. no. :9562097706Email:rkcapricon@EDUCATIONAL QUALIFICATIONSUndergoing Doctor of Philosophy (Ph.D) programme in Aqualife Medicine at Chonnam National University, Republic of KoreaVocational diploma in aquatic veterinary medicine, from IUCEAM board , Republic of KoreaCompleted Veterinary continuing education and professional development (VCEPD) credit in Aquatic veterinary medicine organized by world aquatic veterinary medical association. Certified in animal protection, welfare and research under Article 13 of animal protection act, Republic of Korea on 17th April 2018.Master of Fisheries Science (MFSc) in Aquatic Animal Health Management with OGPA 8.68/10 from ICAR- Central Institute of Fisheries Education, Mumbai, IndiaBachelor of Fisheries Science (BFSc) with OGPA 8.27/10 from Kerala University of Fisheries and ocean Studies, Panangad , Kochi, Kerala, IndiaPlus two with 85% marks under Kerala HSE scheme from Devaswom Board Higher Secondary School, Chengannur ,Kerala, India 10th with 98% marks under SSLC ,from Devaswom Board High School , Chengannur, Alappuzha, Kerala, IndiaDOCTORAL THESISStudies on viral transmission kinetics, early uptake, and role of a novel receptor in NNV neuro-pathogenesis in sevenband grouper, Hyporthodus septemfasciatus.MASTERS DISSERTATIONMolecular cloning and characterization of mitochondrial antiviral signaling protein (MAVS) gene from Lates calcarifer.TRAININGS & CONFRENCES ATTENDEDParticipated in KOFFST international conference-2019 held at Busan, South Korea from 7th to 8th November 2019.Participated in KOSFAS-2019 conference held at Yeosu, South Korea from 9th to 10th May 2019.Participated and delivered oral presentation in Aquaculture 2019 conference held at New Orleans, USA from 6th to 11th March 2019.Participated in KOFFST international conference-2018 held at Busan, South Korea from 1st to 2nd November 2018.Participated in 2018 spring meeting of the Korean society of fish pathology held at Busan, South Korea, from 20th to 21st June 2018.Participated in 26th annual conference of Indian Virological Society, Virocon-2017 organized by NITTE University, Mangalore, India from 7th to 9th December 2017. Participated and delivered oral presentation in 11th Indian fisheries and Aquaculture Forum (IFAF) 2017 organized by ICAR- Central Institute of Fisheries Technology at Cochin from 21st to 24th November 2017Participated in International Symposium on Aquatic Animal Health and Epidemiology for Sustainable Asian Aquaculture (ISAAHE) at ICAR-NBFGR, Lucknow, India from 20th to 21st April 2017.Participated and delivered oral presentation in second student convention on Innovative Approaches for Academic Excellence in Higher Fisheries Education at ICAR- CIFE, Mumbai, India from 4th to 5th March 2017.One month hands on training in molecular disease diagnostics and data analysis at Class III bio-safety Laboratory of ICAR- National Institute of Veterinary Epidemiology and Disease Informatics, Bangalore, India from 1st September 2016 to 30th September 2016.Participated in Pillay Aquaculture Foundation Third Congress on Social Entrepreneurship in aquaculture at ICAR- CIFE, Mumbai, India from 27th to 29th April 2016.Undergone short term training on Development of finfish cell lines for viral disease diagnosis organized by PMFGR, National Bureau of Fish Genetic Resources, and Kochi from 23rd to 28th February 2015. Undergone one month hands-on training in animal cell culture and cytological techniques from 1st to 30th November 2014 at Rajiv Gandhi Centre for Biotechnology, Trivandrum.Participated in World ocean science congress 2015 organized by WOSC consortium on 5th to 8th February, 2015, at Kochi.One day awareness training course on HACCP provided by Intertek on 21st March 2014.Two days Internal Auditor training on FSMS ISO 22000:2005 provided by Intertek on 22nd -23rd March 2014.Participated in the 2013 Undergraduate competition for Alltech’s Young Scientist AwardRESEARCH EXPERIENCESWorking as Research assistant in NRF funded project on Nervous necrosis virus pathogenesis in seven band grouper aquaculture at Chonnam National University, South Korea from March 2nd 2018.Worked as Junior Research Fellow in the DBT funded project on Molecular screening, cell culture-based isolation and characterization of finfish and shellfish viruses and establishment of National Repository at ICAR- Central Institute of Fisheries Education, Mumbai from 9th September 2017 to 13th February 2018.Worked as project trainee at Centre for Aquatic Animal Health, School of Aquaculture and Biotechnology, Kerala University of Fisheries and Ocean Studies, Kerala, India from 14th April to 25th July 2015. Worked on the development of rapid dot gold NP based detection of nitrofuran residues in fish and shellfish.Student investigator under the KSCSTE Student Project scheme for the projects onBioprospecting potential of endophytic bacteria from mangrove environments. (2011-2012)Characterization of pathogenic E. coli isolates associated with seafood. (2014-2015)AWARDS ACHIVEMENTS AND RECOGNITIONBest poster award in fish pathology at 2019 KOFFST international conference held on 7th – 8th November 2019 at Busan, RoK.Excellence certificate for the 2019 school pub meeting organized by college of fisheries and ocean sciences, Chonnam National university, RoKAwarded Overall best MFSc dissertation of ICAR-Central Institute of Fisheries Education, Mumbai, India for the year 2017-18.Awarded the National Research Foundation, Korea (NRF) research support for Pursuing Doctor of Philosophy (Ph.D) programme in Aqualife Medicine.Awarded with Chonnam National University Global Scholarship for Pursuing Doctor of Philosophy (Ph.D) programme in Aqualife Medicine.Best poster award in Animal virology at 26th annual conference of Indian Virological Society, Virocon-2017 organized by NITTE University, Mangalore, India from 7th to 9th December 2017. Awarded Institutional fellowship (2015-2017) for pursuing masters’ degree in aquatic animal health management at ICAR-Central Institute of Fisheries Education, Mumbai, India.Secured first rank in all India level competition of Best Fisheries Graduate of India-2015 and awarded Dr. C. K. Nayak AwardSecured 16th rank in ICAR’s 20th All India PG Entrance Examination 2015Officiated the post of University governing council member of Kerala University of Fisheries and Ocean Studies from March 2012 to January 2015.Recipient of Merit scholarship from Kerala University of Fisheries and Ocean Studies for securing highest overall grade point for undergraduate degree program during the academic year 2010-2015.KCSTE recognized Student Project Fellow from November 2010 to January 2015.PUBLICATIONSJournal ArticlesKrishnan, R., Kim, J.O., Qadiri, S.S.N., Kim, J.O. and Oh, M.J., 2020. Early viral uptake and host-associated immune response in the tissues of seven-band grouper following a bath challenge with nervous necrosis virus. Fish & Shellfish Immunology. Accepted for publication.Qadiri, S.S.N., Kim, S.J., Krishnan, R., Kim, J.O., Kole, S., Kim, W.S. and Oh, M.J., Early uptake of viral haemorrhagic septicemia virus (VHSV) in mucosal tissues of juvenile olive flounder (Paralichthys olivaceus) following an immersion challenge. Aquaculture, 518, pp- 734859.Krishnan, R., Kim, J.O., Kim, J.O., Qadiri, S.S.N., Kim, S.J and Oh, M.J., 2019. Validation of housekeeping genes as candidate internal references for quantitative expression studies in healthy and nervous necrosis virus infected seven band grouper (Hyporthodus septemfasciatus). Fisheries and Aquatic Sciences, 22(1), pp- 28.Whang, I., Kim, Y., Lee, B., Krishnan. R., Shin, S, P., 2019. Dicyema sphyrocephalum (Myxosporea: Sinuolineidae) isolated from Korean common octopus Octopus minor in Korea. Journal of Veterinary Science - under review Krishnan, R., Jeena, K. and Prasad, K.P., 2019. Nervous necrosis virus induced oxidative imbalance and host associated antioxidant response in Asian seabass brain. Aquaculture - submittedKrishnan, R., Qadiri, S.S.N. and Oh, M.J., 2019. Functional characterization of seven-band grouper immunoglobulin like cell adhesion molecule, Nectin4 as a cellular receptor for nervous necrosis virus. Fish & shellfish immunology, 93, pp-720-725.Krishnan, R., Kim, J.O., Kim, J.O., Qadiri, S.S.N., Kim, S.J and Oh, M.J., 2019. Immunoglobulin-like Cell Adhesion Molecules, Nectins - Characterization, functional prediction and expression profiling from seven-band grouper, Hyporthodus septemfasciatus. Aquaculture, 506, pp-387-393.Krishnan, R., Mushtaq, Z., Jeena, K., Vismai N.T and Prasad, K.P., 2019. Interferon-regulatory factors, IRF3 and IRF7 in Asian seabass, Lates calcarifer: Characterization, ontogeny and transcriptional modulation upon challenge with nervous necrosis virus. Fish & shellfish immunology, 89, pp-468-476.Qadiri, S.S.N., Kim, S.J., Krishnan, R., Kim, J.O., Kole, S., Kim, W.S. and Oh, M.J., 2019. Localization and tissue tropism of viral haemorrhagic septicemia virus (VHSV) in experimentally infected juvenile olive flounder, Paralichthys olivaceus: An in situ hybridization and immunohistochemical study.?Aquaculture, 505, pp- 242-252Qadiri, S.S.N., Kim, S.J., Krishnan, R., Kim, J.O., Kim, W.S. and Oh, M.J., 2019. Development of an in-situ hybridization assay using riboprobes for detection of viral haemorrhagic septicemia virus (VHSV) mRNAs in a cell culture model. Journal of virological methods, 264, pp.1-10.Jeena, K., Krishnan, R., Shyam, K.U., Babu, P.G., Lakra, W.S., Purushothaman, C.S. and Prasad, K.P., 2018. Dynamics of Infection in Selected Tissues of White Spot Syndrome Virus-Infected Litopenaeus vannamei. Int. J. Curr. Microbiol. App. Sci, 7(6), pp.3003-3008.Kokkattunivarthil, S., Krishnan, R., Kezhedath, J., Prasad, K.P and Vismai N.T., 2018. New set of PCR primers for SYBR green-based qPCR detection of IMNV in India. Aquaculture, 495, pp.726-730.Krishnan, R., Jeena, K. and Prasad, K.P., 2018. Preliminary investigations on the role of Drp-1 dependent mitochondrial fission in attenuating RLR downstream signaling during nervous necrosis virus infection. Fish & shellfish immunology, 80, pp.618-623.Krishnan, R., Jeena, K., Mushtaq, Z., Shyam, K.U. and Kurcheti, P.P., 2018. Antiviral activity of transiently expressed mitochondrial antiviral signaling adapter, MAVS orthologue from Asian seabass. Fish & shellfish immunology, 76, pp.183-186. Mushtaq, Z., Krishnan, R., Prasad, K.P., Bedekar, M.K. and Kumar, A.P., 2018. Molecular cloning, characterization and expression profiling of galectin-9 gene from Labeo rohita (Hamilton, 1822). Fish & shellfish immunology, 76, pp.287-292.Krishnan, R., Babu, P.G., Jeena, K., Tripathi, G. and Prasad, K.P., 2018. Molecular characterization, ontogeny and expression profiling of mitochondrial antiviral signaling adapter, MAVS from Asian seabass Lates calcarifer, Bloch (1790). Dev. Comp. Immunol., 79, pp.175-185.Prasad, K.P., Shyam, K.U., Banu, H., Jeena, K. and Krishnan, R., 2016. Infectious Myonecrosis Virus (IMNV)–An alarming viral pathogen to Penaeid shrimps. Aquaculture., 477,pp.99-105Krishnan, R., 2014. Probiotic potential of Bacillus species isolated from freshwater fish Anabas testudineus in Labeo rohita. Int. J. Multidisciplinary Res. Devel. 1(1), pp.46-50.AbstractsRahul Krishnan, Jong-Oh Kim, Syed Shariq Nazir Qadiri, Jae-Ok Kim, Yo-Seb Jang and Myung-Joo Oh. Study on nervous necrosis virus (NNV) uptake and associated immune response in gill of seven band grouper after immersion challenge. Book of abstracts, KOFFST international conference 2019, P-414.Syed Shariq Nazir Qadiri, Soo-Jin Kim, Rahul Krishnan, Yo-Seb Jang, Jae-Ok Kim, and Myung-Joo Oh. A time course study on early uptake and trafficking of viral hemorrhagic septicemia virus (VHSV) in mucosal tissues of juvenile olive flounder after waterborne infection. Book of abstracts, KOFFST international conference 2019, P-408Jae-Ok Kim, Rahul Krishnan, Soo-Jin Kim, Syed Shariq Nazir Qadiri, and Myung-Joo Oh. Development of in situ hybridization assay using RNA probes for genome detection of nervous necrosis virus. Book of abstracts, 2019 spring meeting of the Korean society of fish pathology, P-19.Rahul Krishnan, Jong-Oh Kim, Jae-Ok Kim, Syed Shariq Nazir Qadiri , Soo-Jin Kim and Myung-Joo Oh. Characterization of piscine nectin-4 - a potential nervous necrosis virus receptor in seven-band grouper. Book of abstracts, Aquaculture 2019, p- 583Jae-Ok Kim, Soo-Jin Kim, Rahul Krishnan and Myung-Joo Oh. Development of in situ hybridization using rna probe for rna1 segment detection of nervous necrosis virus (NNV), Book of abstracts, Aquaculture 2019, p- 553.Soo-Jin Kim, Jae-Ok Kim, Syed Shariq Nazir Qadiri, Rahul Krishnan and Myung-Joo Oh. Viral hemorrhagic septicemia virus loads in fish organs and blood under VHSV susceptible water temperature, Book of abstracts, Aquaculture 2019, p- 554.Soo-Jin Kim, Jae-Ok Kim, Syed Shariq Nazir Qadiri, Rahul Krishnan and Myung-Joo Oh. investigation to find effective pretreatment method of live VHSV immersion vaccine in juvenile olive flounder, Book of abstracts, Aquaculture 2019, p- 555.Soo-Jin Kim, Jae-Ok Kim, Syed Shariq Nazir Qadiri, Rahul Krishnan and Myung-Joo Oh. Immune-related gene expression levels in organs of live VHSV vaccine immersion treated and VHSV challenged flounder, Book of abstracts, Aquaculture 2019, p- 556. , Book of abstracts, Aquaculture 2019, p- 557.Jae-Ok Kim, Soo-Jin Kim, Rahul Krishnan and Myung-Joo Oh. Development of in situ hybridization using RNA probe for rna2 segment detection of nervous necrosis virus (NNV).Syed Shariq Nazir Qadiri, Soo-Jin Kim, Rahul Krishnan, Jae-Ok Kim, Wi-Sik Kim and Myung-Joo Oh, development of an in-situ hybridization (RNA-ISH) assay using riboprobes for detection of viral haemorrhagic septicemia virus (VHSV) mRNAs in a cell culture model, Book of abstracts, Aquaculture 2019, p- 872.Rahul Krishnan, Jong-Oh Kim, Jae-Ok Kim, Syed Shariq Nazir Qadiri, Soo-Jin Kim and Myung-Joo Oh. Evaluation of candidate reference genes for quantitative expression studies in seven band grouper (Hyporthodus septemfasciatus) tissues following nervous necrosis virus infection. Book of abstracts, KOFFST 2018, p-508.Rahul Krishnan, Jong-Oh Kim, Jae-Ok Kim, Syed Shariq Nazir Qadiri, Soo-Jin Kim and Myung-Joo Oh. Characterization of nectin-4; A potential cell adhesion molecule for pathogenesis of nervous necrosis virus in seven-band grouper. Book of abstracts, KOFFST 2018, p-509. Soo-Jin Kim, Jae-Ok Kim, Syed Shariq Nazir Qadiri, Rahul Krishnan and Myung-Joo Oh. Acquired resistance against VHSV infection in live VHSV immersed olive flounder at 17°C. Book of abstracts, KOFFST 2018, p- 447Jae-Ok Kim, Soo-Jin Kim, Rahul Krishnan, Syed Shariq Nazir Qadiri and Myung-Joo Oh. Investigation of nervous necrosis virus (NNV) replication status using double labeling RNA in situ hybridization method. Book of abstracts, KOFFST 2018, p-448Jae-Ok Kim, Soo-Jin Kim, Rahul Krishnan, Syed Shariq Nazir Qadiri and Myung-Joo Oh. Localization of nervous necrosis virus (NNV) in brain tissue of sevenband grouper (Hyporthodus septemfasciatus) using RNA in situ hybridization method. Book of abstracts, KOFFST 2018, p- 482Syed Shariq Nazir Qadiri, Soo-Jin Kim, Rahul Krishnan, Jae-Ok Kim, Wi-Sik Kim and Myung-Joo Oh. Spatiotemporal localization of viral hemorrhagic septicemia virus (VHSV) using RNAISH assay in an in-vitro model. Book of abstracts, KOFFST 2018, p-506 Syed Shariq Nazir Qadiri, Soo-Jin Kim, Rahul Krishnan, Jae-Ok Kim, Wi-Sik Kim and Myung-Joo Oh. Immunocytochemical detection of viral hemorrhagic septicemia virus (VHSV) in infected FHM cells. Book of abstracts, KOFFST 2018, p- 507Rahul Krishnan, P. Gireesh Babu, Jeena K, Shyam K U, Kurcheti Pani Prasad. Sub-cellular localization and in-vitro antiviral activity of transiently expressed MAVS orthologue from Asian Seabass., Book of Abstracts, Virocon-2017, p-167.Shyam K U, Rahul Krishnan, Jeena K, Kurcheti Pani Prasad. Standardization of SYBR Green chemistry based quantitative real-time PCR assay to detect infectious myonecrosis virus (IMNV)from infected shrimp tissues., Book of Abstracts, Virocon-2017, p-173.Rahul Krishnan, K. Jeena, K. U. Shyam and Kurcheti Pani Prasad. Abrogation of RLR downstream signaling by nervous necrosis virus (NNV) is mediated through Drp-1 dependent mitochondrial fission. Book of Abstracts, IFAF 2017, p- 293-294.Rahul Krishnan., Rahul Rajendran., Devika Pillai., Greeshma S S. Studies on Bacillus mycoides, as potential probiotic, on the haematological and biochemical parameters of Pangasianodon hypophthalmus., Book of Abstracts, IFAF 2017, p-326-327. Rahul Krishnan, Shyam K. U, Jeena K Gayatri Tripathi, Girish Babu. P , K. Pani Prasad, Modulation of mitochondrial morphology and MAVS signaling by betanodavirus in-vitro.,Book of Abstracts ISAAHE- 2017, pp-110Shyam K.U, Jeena K, Pani Prasad K, Rahul Krishnan, Gaurav Rathore, Gayathri Tripathi. A pilot surveillance status of infectious myonecrosis virus in Indian aquaculture. Book of Abstracts ISAAHE- 2017, pp-85.Rahul Krishnan, Muhammed P. Safeena and Sajan George. Isolation and characterization of Shiga toxin producing Escherichia coli, from shucked clam meat. Book of abstracts. World Ocean Science congress 2015, pp- 88-89.Rahul Krishnan, Greeshma S S & Devika Pillai., Immunomodulatory effects of the dietary supplemented Bacillus mycoides on Nile tilapia. Compendium of Abstracts. Kerala Science Congress 2014, pp-251Chapters in book/ training manuals Rahul Krishnan and Shyam KU ( 2019) Infectious Disease Biomarker Discovery through Proteomics – Applications in Aquaculture (Chapter-2)in Emerging Trends in Aquaculture Research, Narendra publishing house, New Delhi , India, PP- 29-48K. Jeena and Rahul Krishnan (2017) Protein expression profiling using protein microarrays. Manual of CAFT Training programme on biotechnological and nanotechnological tools in aquatic animal health management, ICAR-CIFE, Mumbai. pp 200-203.K. Pani Prasad, Bhartendu Vimal, Shyam K. U., Rahul Krishnan (2016). Molecular disease diagnostic techniques. Manual of Winter School Training programme on rapid diagnostics for fish health management, ICAR-CIFE, Mumbai., pp 38-43.K. Pani Prasad, Shyam K. U., Rahul Krishnan (2016). Single Tube Nested RT-PCR for the Semi-quantitative detection of Infectious Myonecrosis Virus. Manual of Winter School Training programme on rapid diagnostics for fish health management, ICAR-CIFE, Mumbai., pp 279-282.Popular articles/extension articlesShameena S S., Vijay Sundar Deva G and Rahul Krishnan. General Principles of Vaccination and Vaccination Strategies in Aquaculture. Aquaculture Times, May-June 2018, p-27Vijay Sundar Deva G, Rahul Krishnan, Shyam K. U and Megha K Bedekar. mRNA Vaccination-A Way Forward for Aquaculture Vaccine Development, Aquaculture Times, May-June 2018, p-41K. U. Shyam, Rahul Krishnan, K. Jeena. Reverse genetics and its forward applications. Aquaculture Times, May-June 2018, p-47REFERENCEProf. Myung-Joo OhProfesssorDepartment of Aqualife MedicineCollege of Fisheries and ocean sciencesChonnam National University Yeosu, Republic of KoreaOhmj@jnu.ac.kr Dr. K. V. RajendranPrincipal Scientist and HeadAquatic Animal Health Management DepartmentICAR- Central Institute of Fisheries EducationMumbai-400061Ph. 08879646750kvrajendran@cife.edu.inDr. K. Pani PrasadPrincipal ScientistAquatic Animal Health Management DepartmentICAR- Central Institute of Fisheries EducationMumbai-400061Ph. 098 67 241101kpaniprasad@cife.edu.inDr. Devika PillaiProfessor and HeadDepartment of Aquatic Animal HealthSchool of Aquaculture and BiotechnologyKUFOS, Kochi, KeralaPh. 9446111033devikamanoj.pillai@ ................
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