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Ms. Day/ Honors Genetics Name __________________________________ Pd: ______
BIOTECHNOLOGY PPT GUIDE #19
PART 1: Introduction to Biotechnology and DNA Plasmids/Gene Cloning
1. What is “biotechnology”?
2. What is a plasmid?
3. Where are plasmids found? _________________________________
4. What is gene cloning?
5. What is the other name for restriction enzymes? ________________________________________
6. How do restriction enzymes work?
7. Where are restriction enzymes found? ________________________________________________
8. What do restriction enzymes do for bacteria in “Nature” (i.e.-in the bacteria)?
9. What is a “restriction site”?
10. What is the difference between a restriction fragment with a “BLUNT” vs one with a “STICKY” end?
11. What is the function of DNA ligase (yes, the same enzyme that is used in DNA replication)?
12. Name 3 different restriction enzymes that science use in DNA technology.
1. ii. iii.
13. Label the following diagram.
[pic]
14. What is a “cloning vector”?
15. What is a “recombinant plasmid” mean?
16. What is a “transformed cell”?
17. What are the steps needed to clone a gene using a plasmid? BE SPECIFIC!
1. ________________________________________________________________________
2. ________________________________________________________________________
3. ________________________________________________________________________
4. ________________________________________________________________________
5. ________________________________________________________________________
6. ________________________________________________________________________
18. When cloning a gene, what molecule that from the above steps is analogous to (acts like):
a. molecular scissors= ______________________________________________
b. molecular glue = ______________________________________________
19. What are the 4 reasons WHY scientists would want to clone a gene?
1. = _______________________________________________________________________
2. =_______________________________________________________________________
3. =_______________________________________________________________________
4. =_______________________________________________________________________
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