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Ms. Day/ Honors Genetics Name __________________________________ Pd: ______

BIOTECHNOLOGY PPT GUIDE #19

PART 1: Introduction to Biotechnology and DNA Plasmids/Gene Cloning

1. What is “biotechnology”?

2. What is a plasmid?

3. Where are plasmids found? _________________________________

4. What is gene cloning?

5. What is the other name for restriction enzymes? ________________________________________

6. How do restriction enzymes work?

7. Where are restriction enzymes found? ________________________________________________

8. What do restriction enzymes do for bacteria in “Nature” (i.e.-in the bacteria)?

9. What is a “restriction site”?

10. What is the difference between a restriction fragment with a “BLUNT” vs one with a “STICKY” end?

11. What is the function of DNA ligase (yes, the same enzyme that is used in DNA replication)?

12. Name 3 different restriction enzymes that science use in DNA technology.

1. ii. iii.

13. Label the following diagram.

[pic]

14. What is a “cloning vector”?

15. What is a “recombinant plasmid” mean?

16. What is a “transformed cell”?

17. What are the steps needed to clone a gene using a plasmid? BE SPECIFIC!

1. ________________________________________________________________________

2. ________________________________________________________________________

3. ________________________________________________________________________

4. ________________________________________________________________________

5. ________________________________________________________________________

6. ________________________________________________________________________

18. When cloning a gene, what molecule that from the above steps is analogous to (acts like):

a. molecular scissors= ______________________________________________

b. molecular glue = ______________________________________________

19. What are the 4 reasons WHY scientists would want to clone a gene?

1. = _______________________________________________________________________

2. =_______________________________________________________________________

3. =_______________________________________________________________________

4. =_______________________________________________________________________

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