PDE4D high expression correlates with ... - Journal of Cancer

Cell migration and invasion assays were performed to test the effect of PDE4D on pancreatic cancer cells PANC1 and Bxpc3, using cell culture inserts with PET membrane (Corning, USA) and BD BioCoat™ Matrigel™ Invasion Chamber (8 μm pore size) (BD Bioscience , USA), respectively. 24 h after cells were transfected with PDE4D siRNAs (si-PDE4D-1 and si-PDE4D-2) or negative control siRNA (siNC), cell migration and invasion assays were performed according to the manufacturer’s protocols and ... ................
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