Introduction to Physiology

Introduction to

Physiology

BIO 130 Laboratory Manual

2017

Dutchess Community College Department of

Allied Health & Biological Sciences (AHBS)

2

BIO 130 Laboratory Manual DCC, AHBS Dept.

Dr. Sandra Fraley Department of Allied Health & Biology Sciences

Dutchess Community College

Copyright ? 2017 by Sandra Fraley, PhD All rights reserved. Permission in writing must be obtained from the author before any part of this work may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopying

and recording, or by any information storage or retrieval system

First printing: Fall 2017

3

Preface

Format of the BIO 130 Lab Manual

The material in this lab manual is designed to encourage discovery-based learning. Each Lab is constructed in alternating fact/question/conclusion format so that concepts are presented in reasoned sequence with statements and questions alternating, leading you to make conclusion about a topic.. After performing lab experiments, you will be asked to draw conclusions based on your interpretation of the results. As much as possible, the manual avoids a step-by-step directional approach. Instead, it is hoped that you can discern and anticipate procedures that will give you controlled experimental results. BIO 130 is a course in which it is you develop your best practices ? both in terms of scientific knowledge and in study habits. It is hoped you will take these with you to success in your future courses.

The manual is organized by semester week. Each week's front page gives the lecture topics, an overview of the class's lab topics, and specific Assignments, if any. The focus of lab activities will generally correspond and relate to lectures topics. Your professor may use the same Assignments or devise his/her own. Assignments may be supplemented with quizzes.

Table of Contents

BIO 130 Course Overview

Required Texts

Assignment summary list

Determination of final grades

Academic Accommodations Student expectations Course description Course objectives Course Student Learning Outcomes Institutional Student Learning Outcomes Tips for Success in Anatomy & Physiology

Week 1

Lecture: Lab 1

Review of Course Topics, Expectations Ch 1 (parts) Ch 2 (specified sections) - Chemistry & Measurements

Lab Rules and Safety procedures Introduction to lab equipment Scientific method; Designing an experiment

Read before class: Ch 1, Section 1.2 ? Scientific Method) Instructional Format for Writing Scientific Reports

- General rules of graphing

Assignment 1 Writing a Scientific Report Due date:

Week 2

Lecture: Ch 4 ? Atoms & Elements; Ch 5 - Radioactivity Lab 2 Measuring Mass with triple beam balance Significant figures and Rounding off

(coordinates with Ch 2, Section 2.3 p31) Common U.S. System conversions (temperature, mass)

(coordinates with Ch 3, Section 3.3 p67)

4

Page

9-

9 9 9 9 10 10 10 11 11 11

13-

14 15 16 17 17

19

20-

21 22 23

Week 3

Lecture: Ch 6 ? Ionic and Molecular Compounds; Ch 7 ? Chemical Quantities and Reactions

Lab 3: Mass/Volume relation Density (coordinates with Ch 2, Section 2.7, pp46-49) Specific Gravity (coordinates with Ch 2, p50)

Assignment 2:

Due Date

Atomic Structure, Electron distribution, Periodic Table

5

26-

27 31 32 33

Week 4

37-

Lecture: Ch 10 (Acids and Bases and Equilibrium)

Lab 4

Pre-lab ? effective ranges of some pH indicators

38

Colorimetric assays for pH

39

Buffers

41

-Assessing Buffer Effectiveness

43

Assignment 3:

Due date:

Graph: Buffers & Buffer Capacity

46

-Instructions for graphing in Excel

Week 5

48-

Lecture: Ch 8 (Gases)

Lab 5:

Gases: Measuring respiratory CO2

49

with pH indicators ? effects of exercise

- Pre-Lab Ch10 ? Chemistry link to Health (Buffers in Blood Plasma),

p353 (the carbonic acid/bicarbonate buffer system)

- Method, Questions & Discussion

Week 6

52-

Lecture: Ch 9 (Solutions)

Lab 6 Preparing % solutions of varying concentrations

53

6

Week 7

57-

Lecture: Ch 11 (parts) - Introduction to Organic chemistry: Hydrocarbons

Ch 12, 14 (parts) Functional groups

Lab 7

Bond type characteristics, polar, nonpolar

58

(coordinates with Ch 6, 11, 13)

Functional groups- formulas & modeling (Ch 12, 14)

59

Week 8

62-

Lecture: Ch 13 ? Carbohydrates

Lab 8:

Carbohydrates: chemical structures and Molecular modeling

63

Colorimetric tests: Benedict's, Iodine tests

66

(glucose, sucrose, starch)

Week 9

70-

Lecture: Ch 15 ? Lipids; Cell membranes and transport

Lab 9 Cell membrane & transport mechanisms

72

-Diffusion: a) in liquid, b) in agar /temperature

74

-c) Osmosis: -using a dialysis membrane

76

-effects of temperature

Assignment 4: Osmosis, effects of temperature (graphing)

78

Due date:

Week 10

79-

Lecture: Ch 16 ? Amino acids, Proteins, and Enzymes

Lab 10

Molecular modeling: amino acid, peptide

80

Enzyme activity ? effect of enzyme concentration (amylase, starch) 80

-Pre-Lab questions

80

-Experiment (Method)

81

Week 11

Lecture: Ch 17- Nucleic Acids and Protein synthesis Lab 11:

Introduction to binocular light microscope use for observation of cells

Identification of microscope parts Image reversal, Field of view, Depth of field `e', `thread' slides Cell nucleus localization (prepared slide) Cell nucleus visualization:

-wet mount (cheek smear) preparation, nucleus stain Practice: 2-min focusing drill (100x)and Grading Rubric

7

83-

84 84 85 86 86 87

Week 12

88-

Lecture: Ch 7 Redox reactions & energy relations (Basic definitions)

Ch 18 (Metabolic Pathways and ATP Production)

Lab 12

Assignment 5 (In Class) - grading sheet

89

-Microscope- Graded two-minute drill for focusing at 100x

Overview of cell structure/function using models (cell, mitochondrion) 90

Osmosis/Toncity - (blood cell in microscope, test tube)

92

(coordinates with Ch 9)

Week 13

97-

Lecture topics Basics of Anatomy : Introduction to the Human Body

see online text: _

Ch 1 at: -Levels of structural organization (atom to organism) -Organ Systems of the Human Body



-Homeostasis (Negative Feedback, Positive Feedback)

:

-Anatomical Terminology

Lab 13

Human Body (human body models-torso, limbs

98-

-Anatomical terminology, organ systems

8

Week 14

103-

Lecture topics

see online text:

Ch 3: Cell Growth and Division at



-Cell Cycle

-Interphase

-Structure of chromosomes (compare to chromatin)

-Mitosis and cytokinesis

Cell division: mitosis followed by cytokinesis

-Phases of mitosis

-Prophase, Metaphase, Anaphase, Telophase

Lab 14

Microscopic examination of Mitosis

104

(whitefish, onion root tip slides)

End-of-semester review (notes page)

108

Week 15

109

Final Exams

 ................
................

In order to avoid copyright disputes, this page is only a partial summary.

Google Online Preview   Download