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Supporting InformationChemodrug-Gated Biodegradable Hollow Mesoporous Organosilica Nanotheranostics for Multimodal Imaging-Guided Low-Temperature Photothermal Therapy/Chemotherapy of Cancer Jianrong Wu,a David H Bremner,b Shiwei Niu,a Menghan Shi,a Haijun Wang,a Ranran Tang,c,* and Li-min Zhua,*aCollege of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai, 201620, PR ChinabSchool of Science, Engineering and Technology, Kydd Building, Abertay University, Dundee DD1 1HG, Scotland, UK cWomen’s Hospital of Nanjing Medical University,Nanjing Maternity and Child Health Care Hospital, Nanjing, 210004, China.*Corresponding author: lzhu@dhu., 13190186401@.Figure S1. Schematic illustration of the fabrication of the Gem-gated hollow mesoporous organosilica nanoplatform (ICG-17AAG@HMONs-Gem-PEG).Figure S2. (a) N2 absorption-desorption isotherms and (b) corresponding pore-size distributions of HMONs, HMONs-CHO, and ICG-17AAG@HMONs-Gem-PEG.Figure S3. Fourier-transform infrared (FT-IR) spectra of different functionalized HMONs.Figure S4. (a) The zeta potential of different functionalized HMONs. (b) DLS-measured hydrodynamic sizes based on the intensity distribution of different functionalized HMONs in water. (c) Hydrodynamic size of ICG-17AAG@HMONs-Gem-PEG dispersed in different solutions for 7 days.Figure S5. UV-Vis absorption spectra of free ICG, 17AAG, gemcitabine and different functionalized HMONs.Figure S6. In vitro ICG and 17AAG release profiles from the ICG-17AAG@HMONs-PEG dispersed in PBS (pH = 7.4).Figure S7. The hydrodynamic size changes of ICG-17AAG@HMONs-Gem-PEG nanoparticles at 25 oC at various pH values.Figure S8. Relative viabilities of MDA-MB-231 cells in vitro after incubation with HMONs and HMONs-CHO nanoparticles at different concentrations.Figure S9. (a) Heating curves of ICG-17AAG@HMONs-Gem-PEG at different concentrations irradiated with a NIR laser (808 nm, 0.5 W cm?2). (b) Heating curves of ICG-17AAG@HMONs-Gem-PEG at the concentration of 50 μg mL?1 with different 808 nm laser powers. (c) Heating curves of ICG-17AAG@HMONs-Gem-PEG (200 μg mL?1) at different pH with 808 nm laser irradiation (0.5 W cm?2). (d) The linear regression curve between cooling stage and negative natural logarithm of driving force temperature of ICG-17AAG@HMONs-Gem-PEG dispersion before or after ICG released.Figure S10. Relative viabilities of MDA-MB-231 cells in vitro after incubation with free ICG and ICG-17AAG@HMONs-Gem-PEG nanoparticles under different laser power irradiation (808 nm).Figure S11. Quantification of ex vivo fluorescence images for different organs (24 h p.i.). H, Li, Ki, Sp, Lu and T = heart, liver, kidneys, spleen, lung, and tumor, respectively.Figure S12. H&E staining of major organs from the control and experimental groups post intravenous injection of ICG-17AAG@HMONs-Gem-PEG with or without NIR irradiation. All scale bars are 50 μm.Figure S13. In vivo toxicology results for mice treated with ICG-17AAG@HMONs-Gem-PEG nanoparticles. (a-d) Serum biochemistry data for (a) Alkaline phosphatase (ALP), (b) Aspartate aminotransferase (AST), (c) Alanine aminotransferase (ALT), (d) blood urea nitrogen (BUN) levels. (e-h) Complete blood counts: (e) Hemoglobin (HGB), (f) Hematocrit (HCT), (g) Mean corpuscular volume (MCV), (h) Blood platelet levels (PLT), (i) White blood cell level (WBC), (j) Red blood cells (RBC), (k) Mean corpuscular hemoglobin (MCH), (l) mean corpuscular hemoglobin concentration (MCHC). (n= five mice per data point).Table S1Pharmacokinetic parameters of ICG after intravenous injection (n = 4).ParametersFree ICGICG-17AAG@HMON-PEGICG-17AAG@HMON-Gem-PEGCmax (μg/mL)38.4 ± 1.152.3 ± 0.7277.5± 0.72AUC0-∞(μg/mL?h)8.67 ± 1.2384.16 ± 14.35*149.8 ± 14.35*MRT (h)　1.27 ± 0.2612.14 ± 1.32*14.56 ± 1.32* ................
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