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Briefly, 125I-labeled CCL18 was incubated with 6 ( 105 cells resuspended in binding buffer (50nM HEPES, pH 7.2, 1 mM CaCl2, 5mM MgCl2, 0.5% BSA) in the presence or absence of unlabeled chemokines. After incubation at room temperature for 30 min, the cells were pelleted through a PBS cushion with 10% sucrose for 1 min at 10,000 ( g. ................
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