Optical Microscope; • Scanning Electron Microscope (SEM ...
Lecture 3 Brief Overview of Traditional Microscopes
? Optical Microscope; ? Scanning Electron Microscope (SEM); ? Transmission Electron Microscope (TEM); ? Comparison with scanning probe microscope (SPM)
General philosophy
Human beings use two kinds of means to gauge objectives: Seeing --- through eyes (light) optical microscope electronic microscope (higher resolution due to short wavelength); Touching --- through hands (probe) SPM.
Converting wavelength (nm) to electron energy (eV): 1240.7/(nm), for example, a 500 nm light corresponds to 1240.7/500 = 2.48 eV
Principles of an optical microscope
Development of microscope takes us back to 400 years ago
Robert Hooke's "Micrographia" (1665)
A hair knot
Room dusts
Red blood cell
Flu virus
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