San Diego Miramar College



|DESCRIPTIVE CHART |

|Student |Student |Unknown |

|Name: |Number: |Number: |

|MORPHOLOGICAL CHARACTERISTICS |PHYSIOLOGICAL CHARACTERISTICS |

|Gram reaction: POSITIVE or NEGATIVE: |CARBOHYDRATE METABOLISM |

|Cell Shape: |O/F glucose, oxidative |O/F glucose, fermentative |

|Arrangement: |Starch hydrolysis |

|Size: |FERMENTATION (acid / gas) |

|Spores: POSITIVE or NEGATIVE |Glucose |

|Motility: MOTILE or NON-MOTILE |Mannitol |

|Capsules: POSITIVE or NEGATIVE |Lactose |

|Acid-fast: POSITIVE or NEGATIVE |CATABOLIC REACTIONS |

|CULTURAL CHARACTERISTICS |Casein hydrolysis |

|Nutrient Agar Plate Growth: |Phenylalanine deamination |Gelatin hydrolysis |

|Colony shape and margin: | | |

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|Colony color: | | |

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|Colony size: | | |

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|Colony elevation: | | |

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|Growth on Agar Slant: | | |

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|Growth in Nutrient Broth: | | |

|Surface: | | |

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|Sediment: | | |

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|Turbidity: | | |

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|Oxygen Requirement: | | |

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|Optimum Temperature: 37°C or 25°C | | |

| |Hydrogen sulfide production |Urea hydrolysis |

| |Ornithine decarboxylase |Lysine decarboxylase |

| |Fat hydrolysis |

| |RESPIRATION |

| |Oxidase |

| |Catalase |

| |Nitrate reduction |

| |IMViC |

| |Indole |MR |

| |VP |Citrate |

|LITMUS MILK | |

|Acid | | |Alkaline | | |

|Coagulation | | |Reduction | | |

|Peptonization | | |Curd | | |

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