PNAS



Table S1. Bacterial strains used

|Strain or plasmid |Description* |Source or reference |

|E.coli | | |

|DH5α |F-, ϕ80dlacZΔM15Δ(lacZYA-argF)U169 deoR recA1 endA1 hsdR17(rk-, mk+) phoA supE44 |Invitrogen |

| |λ- thi-1 gyrA96 relA1 | |

|S17-1 |recA pro hsdR RP4-2-Tc::Mu-Km::Tn7 |(1) |

|P. aeruginosa | | |

|PAO1 |Wild-type |(2) |

|PAO-MW1 | |[pic](3) |

|BE171 |Soil isolate, ATCC 14886 |S. Lory [pic](4) |

|BE171-II |BE171 Δ lasI::FRT ΔrhlI |This study |

|BE173 |Air isolate from a glass-crusher air plate, ATCC 25324 |S. Lory [pic](4) |

|BE173-II |BE173 Δ lasI::FRT ΔrhlI |This study |

|BE177 |Biofilm isolate from an industrial water system, ATCC 700888 |S. Lory [pic](4) |

|BE177-II |BE177 Δ lasI::FRT ΔrhlI |This study |

|PaE2 |Tomato plant |[pic](5) |

|PaE2-II |PaE2 Δ lasI ΔrhlI |This study |

|CI27 |Isolate from a cystic fibrosis chronic infection |J. Burns |

|CI27-II |CI27 Δ lasI ΔrhlI |This study |

|CIG1 |Isolate from a cystic fibrosis chronic infection |A. Bruce Montgomery |

|CIG1-II |CIG1 Δ lasI ΔrhlI |This study |

|Plasmids | | |

|pEXG2 |Gene-replacement vector; sacB Gmr |[pic](6) |

|pEX18-Tc |Gene-replacement vector; sacB Tcr |(7) |

|pPS858 |Source of Gmr-GFP-FRT cassette; Apr, Gmr |(7) |

|pFLP2 |Source of Flp recombinase; Apr/Cbr |(7) |

*Km, kanamycin resistance; Apr, ampicillin resistance; Cbr, carbenicillin resistance; Tcr, tetracycline resistance; Gmr, gentamicin resistance.

References

1. Simon R, Preifer U, Puhler A (1983) A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in gram negative bacteria. Nat. Biotechnology 1:784-91.

2. Holloway BW (1955) Genetic recombination in Pseudomonas aeruginosa. J Gen Microbiol 13(3):572-81.

3. Whiteley M, Lee KM, & Greenberg EP (1999) Identification of genes controlled by quorum sensing in Pseudomonas aeruginosa. Proc Natl Acad Sci U S A 96(24):13904-9.

4. Ernst RK, D'Argenio DA, Ichikawa JK, et al. (2003) Genome mosaicism is conserved but not unique in Pseudomonas aeruginosa isolates from the airways of young children with cystic fibrosis. Environ Microbiol 5(12):1341-9.

5. Wolfgang MC, Kulasekara BR, Liang X, et al. (2003) Conservation of genome content and virulence determinants among clinical and environmental isolates of Pseudomonas aeruginosa. Proc Natl Acad Sci U S A 100(14):8484-9.

6. Rietsch A, Vallet-Gely I, Dove SL, & Mekalanos JJ (2005) ExsE, a secreted regulator of type III secretion genes in Pseudomonas aeruginosa. Proc Natl Acad Sci U S A 102(22):8006-11.

7. Hoang TT, Karkhoff-Schweizer RR, Kutchma AJ, & Schweizer HP (1998) A broad-host-range Flp-FRT recombination system for site-specific excision of chromosomally-located DNA sequences: application for isolation of unmarked Pseudomonas aeruginosa mutants. Gene 212(1):77-86.

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