BIO5
The sequence of the forward primer and the probe are identical to the top strand (5’ to 3’). All sequences from the NCBI database show only the 5’ to 3’ sequence. The reverse primer binds to the 5’ to 3’ strand (top) of the product of reverse transcribed cDNA of SARS CoV2 but is resembles the bottom strand (3’to 5’). ................
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