DETERMINATION OF DNA CONCENTRATION AND PURITY ¶ ¶ STUDENT ...
dilute the DNA 1/10 and 1/100 and run both dilutions on the gel with a molecular weight marker (MWM) of known concentration. The DNA concentration is determined by comparing it to one band in the MWM that most resembles it in brightness. See Figure 4 for a sample calculation. Figure 3. Bands of chromosomal DNA previously incubated with ethidium ... ................
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