Pakistan Journal of Biological Sciences

OPEN ACCESS

Pakistan Journal of Biological Sciences

ISSN 1028-8880 DOI: 10.3923/pjbs.2019.220.225

Research Article Optimization of Graphene Oxide-based Quencher-free Molecular Beacon for Meat Product Authentication

1,2Hari Widada, 1Abdul Rohman, 1Riris Istighfari Jenie and 1Sismindari

1Faculty of Pharmacy, Universitas Gadjah Mada, Yogayakarta, Indonesia 2School of Pharmacy, Faculty of Medical and Health Sciences, Universitas Muhammadiyah Yogyakarta, Yogyakarta 55183, Indonesia

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Fluorescence intensity

150

100

50

0 0

-50

-100

-150

y = -20.088x+90.046 R2 = 0.901

2

4

6

8

10

Concentration of GO (?g mLG1)

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Fluorescence intensity

450 400 350 300 250 200 150 100

50 00

10 20 30 40 50 60 70 80 90 Temperature (?C)

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Fluorescence intensity

350

300

250

200

150

100

50

0

0

2

4

6

8

10

12

Time (min)

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Fluorescence intensity

8,000

7,000

6,000

5,000

4,000

3,000

2,000

1,000

0.0

6.0

6.5

7.0

7.5

8.0

8.5

9.0

9.5

pH

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Fluorescence intensity

5,000 4,500 4,000 3,500 3,000 2,500 2,000 1,500 1,000

500 0 0

20

40

60

80

100

120

Concentration of NaCl (mM)

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Fluorescence intensity

250 202.83

200

150

100

50

0 Porcine

-50

-100

101.01 Cow

151.79 Goat

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