Pakistan Journal of Biological Sciences
OPEN ACCESS
Pakistan Journal of Biological Sciences
ISSN 1028-8880 DOI: 10.3923/pjbs.2019.220.225
Research Article Optimization of Graphene Oxide-based Quencher-free Molecular Beacon for Meat Product Authentication
1,2Hari Widada, 1Abdul Rohman, 1Riris Istighfari Jenie and 1Sismindari
1Faculty of Pharmacy, Universitas Gadjah Mada, Yogayakarta, Indonesia 2School of Pharmacy, Faculty of Medical and Health Sciences, Universitas Muhammadiyah Yogyakarta, Yogyakarta 55183, Indonesia
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Fluorescence intensity
150
100
50
0 0
-50
-100
-150
y = -20.088x+90.046 R2 = 0.901
2
4
6
8
10
Concentration of GO (?g mLG1)
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Fluorescence intensity
450 400 350 300 250 200 150 100
50 00
10 20 30 40 50 60 70 80 90 Temperature (?C)
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Fluorescence intensity
350
300
250
200
150
100
50
0
0
2
4
6
8
10
12
Time (min)
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Fluorescence intensity
8,000
7,000
6,000
5,000
4,000
3,000
2,000
1,000
0.0
6.0
6.5
7.0
7.5
8.0
8.5
9.0
9.5
pH
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Fluorescence intensity
5,000 4,500 4,000 3,500 3,000 2,500 2,000 1,500 1,000
500 0 0
20
40
60
80
100
120
Concentration of NaCl (mM)
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Fluorescence intensity
250 202.83
200
150
100
50
0 Porcine
-50
-100
101.01 Cow
151.79 Goat
Chicken -57.74
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