Dr



Dr. Bhaskar Godugu

Research Assistant Professor

Department of Chemistry, University of Pittsburgh

Pittsburgh, PA-15260

Phone: 336-554-4903 (Cell), 412-624-8406 (Office)

Email: bgboss1999@, bhg5@pitt.edu

SKILLS

Instruments handled

• Waters Q-Tof Ultima API-US interfaced with 2695 speration Madule

• Shimadzu LCMS-2020

• Shimadzu GCMS-QP5050

• Thermo Finnigan LC-Q and LT-Q Advantage LC-Ion Trap MS

• Agilent technology LC-Quadrupole Time-Of-Flight (Q-Tof) MS

• Applied bio systems Q-STAR XL Q-Tof MS

• MICROMASS QUATTRO – LC Triple Quadrupole MS

• KOMPACT KRATOS MALDI SEQ – TOF MS

• VG – AUTOSPEC M Triple Sector MS

• AGILENT GC-MS 6890/5973

• Well versed with routine maintenance of these mass spectrometers, viz. Source cleaning and reassembling

Techniques

• Nanospray Ionization

• Electrospray Ionization

• Matrix Assisted Laser Desorption Ionization

• Atmospheric Pressure Chemical Ionization

• Liquid Secondary Ion Mass Spectrometry

• High Resolution Mass Spectrometry

• Gas Chromatography-Mass Spectrometry

• Liquid Chromatography-Mass Spectrometry

• Electron Ionization

• Chemical Ionization

• MS/MS (parent, daughter, neutral loss, neutral gain, MIKES, SIR, MRM, data dependent scans)

Proteomics

• Experience on plasma precipitation to separate the proteins from lipids.

• Hands-on experience on plasma, simple and complex protein digestion by using denaturation, reduction, alkylation and promega trypsin

• Protein and peptide sample preparation and clean up methods (Zip tip cleanup) for mass spectrometry based experiments

• Method development for shotgun proteomics by using HPLC/MS platforms(nanoflow LC-MS/MS)

• Quatification studies by adding the isotopic internal standards to digestion mixtures

• Development of the data dependent experiments on LC-ESI-MS/MS platforms for characterizing the protein, peptide sequencing and post-translational modification site determination with major emphasis on oxidation, methylation and deamidation

• Extensive experience on data analysis by using NIST pipeline and MASCOT search

Small molecule mass spectrometry

• Method development for separation and characterization of mixture of small compounds

• Quantification of small molecules by using MRM, SRM methods in Mass spectrometry instruments

• Structural analysis of compounds by using different mass spectrometry techniques

• Extensive experience on interactions of metal ions and biological molecules in the gas phase and various metal ion binding strengths of biological molecules

• Differentiation of positional, geometrical and chiral isomers by using mass spectrometric techniques

Synthesis

• Enough experience on peptide synthesis by using the peptide synthesizer

• Experience on small organic molecule synthesis (one step and two step chemical reactions)

Computer Skills

• Experianced with different instrument software systems i.e., Masslynx, Xcalibur, Analyst and MassHunter

• Basic computer knowledge i.e., Windows – XP, Windows - VISTA, MS-Office, Sigma plots, Chemdraw etc

EXPERIENCE

Research Assistant Professor December 2010 to present

Dept., of Chemistry, UPITT

• In charge of Mass spec facility and day-to-day operation and maintenance of six different kind of instruments, supervision of Teaching graduate assistants, training to mass spec users, sample analysis and data delivery.

Guest Research Scholar 2008 to Nov, 2010

NIST, MD, USA

• Protein (BSA, BCA2, Cyt C and chicken egg yolk) in solution digestion (Reduction, Alkylation and tryptic digestion) and develop the methods to analysis the digested solution in LC-MS (Thermo finnigan LTQ instrument) to look the peptide identifications, protein scores. Also check the spectrum repeatability and reproducibility by changing the Experimental and instrumental conditions such as temperature, pH of the digestion solution, chemical Processing agents, digestion times, spray voltage, tube lens voltage etc. and also check the intensity distribution of peptide ions with changing the conditions.

• And I was also responsible to add quality MS and MS/MS spectra in to NIST mass spectral library.

Doctoral Research 2004 to 2007

NCMS, IICT, INDIA

• My doctoral research is focused on Mass spectral studies of biologically important compounds and their interaction with cations and anions using soft ionization techniques like ESI, MALDI, APCI, and CI.

Teaching 1999 to 2003

Junior & Degree College

• Teaching general chemistry (organic, physical and inorganic) to under graduate students and organic chemistry, Analytical chemistry to graduate students.

JOURANL REVIEWER

• Current Medicinal Chemistry

• Journal of Mass spectrometry

• Analytical Chemical Acta

FELLOWSHIPS

• Guest Research Fellowship, NIST, Gaithersburg, MD, USA (2008)

• Senior Research Fellowship, University Grant Commission (UGC), New Delhi, India (2006)

• Junior Research Fellowship, UGC, New Delhi, India (2004)

PUBLICATIONS

1. Electrospray Tandem Quadrupole Fragmentation of Norfloxacin and Related Ions. On the reversibility of water loss from protonated ions

Pedatsur Neta, Bhaskar Godugu, Yuxue Linag, Yamil Simon-Manso, Xiaoyu Yang and Stephen E. Stein

Journal of American Society for Mass spectrometry,2010.

2. Effect of N- Terminal Glutamic Acid and Glutamine on Mode of fragmentation of diprotonated peptide ions

Bhaskar Godugu, Pedatsur Neta, Yamil Simon-Manso and Stephen E. Stein

Journal of American Society for mass spectrometry, 2010; 21: 1169-1176.

Chiral discrimination of (-amino acids by the DNA tetranucleotides under electrospray ionization conditions

T. Sivaleela, M. Ravi Kumar, S. Prabhakar, G. Bhaskar and M. Vairamani

Rapid Commun. Mass Spectrom., 2008; 22: 204-210.

3. Differentiation of diastereomeric N-aryl tetrahydropyrano/

tetrahydrofurano chromenylamines under electron ionization and

chemical ionization conditions

G.S. Ramanjaneyulu, S. Prabhakar, G. Bhaskar, A.Venkatesham, K. Nagaiah, R. Nageswara Rao, Y. Soujanya and G. Narahari Sastry

Rapid Commun. Mass Spectrom., 2007; 21: 3511-3519.

4. Differentiation of diastereomeric conduramine derivatives under electron ionization and chemical ionization mass spectral conditions

G.S. Ramanjaneyulu, S. Prabhakar, G. Bhaskar, M. Vairamani, J.S. Yadav, Ch.V.S.R. Murty, Y. Soujanya and G. Narahari Sastry

Rapid Commun. Mass Spectrom., 2007; 21: 579-588.

5. Mass Spectral differentiation of diastereomeric hydroxybrevicomins by electron ionization

G.S. Ramanjaneyulu, S. Prabhakar, G. Bhaskar, M. Vairamani, D. Naveen Kumar and B. Venkateswara Rao

Rapid Commun. Mass Spectrom., 2006; 20: 2990-2994.

6. Mass spectral studies of meso-dialkyl, alkyl aryl and cycloalkyl calix(4)pyrroles under positive and negative ioin electrospray ionization conditions

G. Bhaskar, S. Prabhakar, G.S. Ramanjaneyulu, M. Vairamani, V.N.V. Srinivasu and K. Srinivas

J. Mass Spectrom., 2007; 42: 1194-1206.

7. Matrix-assisted laser desorption/ionization studies on transition metalcomplexes of benzimidazolethiosemicarbazones.

G. Bhaskar, S. Prabhakar,N. Prasada Raju and G.S.R. Ramanjaneyulu

Eur. J. Mass Spectrom., 2007; 13: 135-145.

8. Characterisation of a series of acetals/ketals of bis(2-nitrophenyl) ethanediol and bis(4,5-dimethoxy-2-nitrophenyl) ethanediol under APCI mass spectrometric conditions

G. Bhaskar, S. Prabhakar, M. Ravi Kumar, Ch. V. Narsimhaji, K. Srinivas and M. Vairamani

J. Mass Spectrom., 2006; 41: 1608-1614.

9. Differentiation of isomeric substituted diaryl ethers by electron ionization and chemical ionization mass spectrometry

G. Bhaskar, S. Prabhakar, A. Venkat Narsaiah, K. Nagaiah, G.S. Ramanjaneyulu and M. Vairamani

Eur. J. Mass Spectrom., 2006; 12: 101-110.

10. Electrospray ionization studies of transition-metal complexes of 2-acetylbenzimidazolethiosemicarbazone using collision-induced dissociation and ion-molecule reactions

G. Bhaskar, M. Adharvana Chary, M. Kiran Kumar, K. Syamasundar, M.Vairamani and S. Prabhakar

Rapid Commun. Mass Spectrom., 2005; 19: 1536-1544.

POSTER/SEMINAR PRESENTED

Single Protein Digest Spectral Libraries: A New Tool for Quality Control in Proteomics

Qian Dong; Bhaskar Godugu; Lisa E. Kilpatrick; Yuxue Liang; Pedatsur Neta; Xinjian Yan ; Stephen Stein

[Poster presenting in 59th ASMS conference in Denver, Colorado held during June 5 to 9, 2011]

Variations of Tryptic Peptide Yields in the Digestion of Proteins

Stephen Stein; Bhaskar Godugu; Lisa E. Kilpatrick; Yuxue Liang; Pedatsur Neta; Xinjian Yan

[Presentation in Clinical Proteomics Technology for Cancer Annual meeting in

Bethesda, Maryland during September 8-10, 2010]

Differentiation of confiqurational isomers of para-hydroxy phenyl methyl calyx(4)pyrroles by ESI Mass Spectrometry

Dinesh Kumar Ch; Bhaskar Godugu; Naresh Chary V; Santhosh R.P; Srinivas K; Prabhakar S.

[Poster presented in RASSI conference in Hyderabad, AP-INDIA during June, 2010]

Improvement and Application of Analytical Software for Examining Reproducibility in Shotgun proteomics

Xinjian Yan, Dmitrii V. Tchekhovskoi, Bhaskar Godugu, Stephen Stein

[Poster presented in 58th ASMS conference in Salt Lake City, Utah during May 23-27, 2010]

Creating Consensus Spectra with a Peak Density Based Clustering Method

Songfeng Wu, Bhaskar Godugu, Xiaoyu Yang, Steve Stein

[Poster presented in 58th ASMS conference in Salt Lake City, Utah during May 23-27, 2010]

Metrics for LC-MS/MS Performance in Proteomics

Qina Dong, Bhaskar Godugu, Lisa Kilpatrick, Yuri Mirokhin, Pedatsur Neta, Paul Rudnick, Dmitri Tchekhovsoi, Eric Yan, Stephen Stein

[Poster presented in Clinical Proteomics Technologies for Cancer Annual meeting in Bethesda, Maryland during October 5-7, 2009]

Identifying Metrices for digestion Effectiveness

Amy-Joan L. Ham, Corbin W. Whitwell, Lisa Kilpatrick, Lisa J. Zimmerman, Dmitri Tchekhovskoi, Xinjian Yan, Pedatsur Neta, Bhaskar Godugu, Daniel C. Ciebler, Stephen Stein

[Poster presented in Clinical Proteomics Technologies for Cancer Annual meeting in Bethesda, Maryland during October 5-7, 2009]

Extraction and Use of Ion Intensity Data for Examining Reproducibility in Shotgun Proteomics

Xinjian Yan, Dmitrii Tchekhovskoi, Bhaskar Godugu, and Stephen Stein

[Poster presented in 57th ASMS conference in Philadelphia, PA during May 31- June 4, 2009]

Dissociation of [M+H]+, [M+Na]+ and [M-H]- ions of meso dialkyl, a lkyl aryl, and cycloalkyl calix(4)pyrroles under ESI conditions. G. Bhaskar, K. Srinivas, G.S. Ramanjaneyulu and S. Prabhakar [paper presented in 11th ISMAS-WS on mass spectrometry, Goa, India, held during March 25-30, 2007]

Characterisation of a series of acetals/ketals of bis(O-nitrophenyl)etahnediol and bis(4,5-dimethoxy-2-nitrophenyl)etahnediol under negative ion atmospheric pressure chemical ionisation (APCI) mass spectral condition

G. Bhaskar, M. Ravi Kumar, S.Prabhakar, M. Vairamani, Ch. Venkata Narsimhaji

[Poster presented in 10th ISMAS Triennial International Symposium on Mass Spectrometry. Tea County, Munnar, Kerala, India, held during January 28th to February 1st, 2006]

EDUCATION

Ph.D Chemistry, March 2008

Indian Institute of Chemical Technology (IICT), Hyderabad (India)

Thesis Title: “Mass spectral studies of biologically important compounds and their interaction with cations and anions using soft ionization techniques”.

M.Sc Chemistry, April 1999

University of Hyderabad, Hyderabad, A.P., India

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