Calculating a pH slope percentage - Hanna Inst
Calculating a pH Slope Percentage
Calculating a pH slope percentage verifies that your pH probe is functioning properly.
Generally a slope between 85 and 105% and an offset of ?30 mV is acceptable. Outside of this range, you may need to replace your pH probe.
To calculate the slope percentage of your pH probe:
1) Measure and record the mV value in pH 7.0 buffer; this is the electrode offset. 2) Measure the mV value in a second buffer, such as pH 4.0. 3) To determine the electrode slope, calculate the absolute mV difference in between the two buffers. 4) Divide this by the difference of pH units between buffers. (Example: the difference in pH units between
7.01 buffer and 4.01 buffer is 7.01-4.01=3). 5) To convert this result to electrode slope percentage, divide the electrode slope by the theoretical maximum slope
(59.16 mV/pH unit @ 25?C), and multiply by 100.
% slope = (mV / pH units) * 100 (59.16 mV / pH units)
Sample Calculation
Electrode 1 generates -15 mV in pH 7.01 and +160 mV in pH 4.01. Absolute mV difference: +160 mV ? (-15 mV) = +175 mV
% slope =
(175 mV / 3 pH units) (59.16 mV / pH units) * 100 =
58.33 59.16
* 100 = 98.6%
Electrode 2 generates +15 mV in pH 7.01 and +160 mV in pH 4.01. Absolute mV difference: +160 mV ? (+15 mV) = +145 mV
% slope =
(145 mV / 3 pH units) (59.16 mV / pH units)
* 100 =
48.33 59.16
* 100 = 81.7%
Conclusion: Both have acceptable offsets. Electrode 1 has an offset of -15 mV and a slope of 98.6% while electrode 2 has an offset of +15 mV and a slope of 81.7%. Electrode 1 is working properly while electrode 2 has an unacceptable slope. If changing the fill solution, cleaning the electrode, and calibrating does not help, replace the electrode.
Calculating a pH Slope Percentage
?Hanna Instruments
Calculating a pH Slope Percentage
pH electrode calibration
Calculating a pH Slope Percentage 4/2017 PRINTED IN USA
Click here to watch our video: how to check the slope and offset of a pH meter (3 minutes)
Calculating a pH Slope Percentage
?Hanna Instruments
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