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Purity of primary neutrophils was determined as CD11b and Ly-6G double-positive cells in FACS analysis. Purified neutrophils were stimulated with PGE 2 (10 M, Nacalai Tesque, Inc., Kyoto, Japan), the EP2 agonist, ONO-AE1-259 (0.5 M), dibutyryl cAMP (100 M, Sigma), TNF- (1 ng/ml, R&D) as indicated in Results or Figure Legends in the presence of ... ................
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