Use of SDS -Page gel for protein.



Acrylamide mix ( Acrylamide and bisacrylamide)

TEMED(N,N,N’,N;-tetramethylethylnediamine)

Sodium Dodecyl sulfate (SDS)Hazardous Substances Policy - Assessment

CHEMICAL HAZARD AND RISK ASSESSMENT

School of Biosciences

|Name of |Dr.Robert Insall | | |Assessment Number* |2032 |

|supervisor | | | | | |

| | Signature | | | |

|Assessor |Soha Zouwail | | |Date of Assessment |17/2/02 |

Signature

Notes A School COSHH form in Word is available on the School Server.

Available from the Health and Safety Unit.

Guidance on making an assessment is given in Making a Chemical Hazard and Risk Assessment.

Guidance is also available from Guidance on Completing the Chemical Hazard and Risk Assessment Form.

Use a continuation sheet to expand any section of this form in hard copy version.

|1 | |LOCATION OF THE WORK ACTIVITY | |8th Floor labs |

| | | | | |

|2 | |PERSONS WHO MAY BE AT RISK |

| | | | |

|List names where possible |Frank Zimmerman,Jason King , Margarita Tchibalina,Robert Insall ,Soha Zouwail, and may be other members of both |

| |Robert’s &Laura’s labs |

| |

| |

|3 | |ACTIVITY ASSESSED | |Use of SDS -Page gel for protein. |

| | | | | |

| | |MATERIALS INVOLVED |Attach copies of data sheet(s) | |

|NAME |AMOUNT** |HAZARD |RISK PHRASES |HAZDAT |BIOSCIENCESNO*** |

| | | | |NO*** | |

|Acrylamide mix |4 ml of 30% Acrylamide|Toxic& |R39/27Very toxic:danger of | | |

|( Acrylamide and bisacrylamide) |mix |Carcinogenic |irreversible effects in contact | | |

| | | |with skin | | |

| | | |R23/24/25 Toxic by inhalation ,in | | |

| | | |contact with the skin &if swallowed| | |

| | | | | | |

| | | |R45 may cause cancer | | |

| | | |R46 may cause heritable genetic | | |

| | | |damage | | |

| | | |R 46 Danger of serious damage to | | |

| | | |health by prolonged exposure | | |

|TEMED(N,N,N’,N;-tetramethylethyl |4 ul |Irritant |R11 highly flammable | | |

|nediamine) | | |R20/22 harmful by inhalation or if | | |

| | | |swallowed | | |

| | | |R34 causes burns | | |

| | | |R 36/37/38 Irritant to eyes | | |

| | | |,respiratory system &skin | | |

|Sodium Dodecyl sulfate (SDS) | 0.1 ml of 10% |Irritant |R41 risk of serious damage to eyes | | |

| |solution |Harmful if swallowed |R42 may cause sensitisation by | | |

| | | |inhalation. | | |

| | | |R20/22 harmful by inhalation &if | | |

| | | |swallowed | | |

| | | |R36/37/38 irritation to eyes, | | |

| | | |respiratory system, &skin. | | |

|Ammonium persulphate (APS) |0.1 ml of 10% APS |Irritant |R8 contact with combustible | | |

| | |Harmful if swallowed |material may cause fire. | | |

| | | |R22 harmful if swallowed. | | |

| | | |R34 causes burns. | | |

| | | |R42/43 may cause sensitisation by | | |

| | | |inhalation or skin contact. | | |

|Hydroxymethylaminomethane(Tris) |2.5 ml of 1.5 M Tris |Irritant |R36/37/38 Irritant to eyes, | | |

| | | |respiratory system, &skin. | | |

|5 | |INTENDED USE |

| | | | |

|Give brief details and attach protocol/instructions |Preparation & use of gels containing different percentages of acrylamide to separate proteins |

| |of different sizes. |

|6 | |RISKS to HEALTH and SAFETY from INTENDED USE |

| | | | |

|From personal exposure or hazardous reactions. Refer to OELs, flash points, etc., as appropriate. Are pregnant women, breast-feeding mothers especially|

|at risk? |

|There is risk of inhalation of powder when preparing SDS solution. There is risk of skin contamination when using acrylamide mix, or skin burn when |

|using the TEMED & APS solutions. |

|7 | |CONCLUSIONS ABOUT RISKS |

| | | | |

|Is level of risk acceptable? Can risk be prevented or reduced by change of substance/procedure? Are control measures necessary? |

|The level of risk is not acceptable and control measures should be followed. |

|8 | |CONTROL MEASURES |

| | | | |

|Additional to Good Chemical Practice | |

|The sodium dodecyl sulphate (SDS) should be weighed in the fume hood on the 7th floor whilst wearing mask, gloves and lab coat. |

| The acrylamide mix &TEMED should be all used while wearing gloves. |

|9 | |INSTRUCTION/TRAINING |

| | | | |

|Specify course(s) and/or special arrangements. | |

|Personnel involved in the clean up procedure must be adequately trained and experienced in handling carcinogenic materials. |

|10 | |MONITORING |

| | | | |

|Performance of control measures, | |

|Check fume hood is working properly before use. |

| |

|Personal exposure | |Health Surveillance | |

|Wearing lab coat and gloves during the whole procedure. | |

| | |

|11 | |WASTE DISPOSAL PROCEDURE |

| See School Server for Approved Procedure Document on specific Chemical Waste Disposal. |

|Polymerised Acrylamide can be placed in yellow bags for incineration. Any of the other chemicals could be disposed in the drain followed by large amount|

|of water. |

|12 | |REVIEW |

| | | | |

|Enter the date or circumstances for review of assessment (maximum review interval 5 years) | |

|February 2007 |

|13 | |EMERGENCY ACTION |

| | | | |

|TO CONTROL HAZARDS |To stabilize situation eg spread absorbant on liquid spill; eliminate sources of ignition, etc. |

|Any spillage of acrylamide should be carefully cleaned whilst wearing gloves and protective clothes and the waste should be discarded as special waste |

|(contact Brian Facer in stores). Treat contaminated surface with household bleach and then wash thoroughly with water. If APS or TEMED has been |

|spilled, sources of ignition and combustible material should be eliminated & absorbant material should be spread on the spill. |

| |

| |

|TO PROTECT PERSONNEL |Evacuation, protection for personnel involved in clean-up, Special First Aid | |

|If any of the hazardous chemicals comes in contact with the skin, remove people from source of contamination, bring injured person under a shower, wash |

|contaminated part of the body for a minimum of 10 minutes, remove any contaminated clothing and seek medical advice. Ensure that the person involved in |

|the clean up is wearing gloves and lab coat. Breathing apparatus may also be required. |

|TO RENDER SITE OF EMERGENCY SAFE |Clean-up/decontamination | |

|For air contamination: evacuate the place and ventilate room as much as possible to dispel vapour. |

|For flammable materials: shut off all sources of ignition in the room, evacuate all people immediately, contact security or ring fire alarm. |

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| CONTACT PHONE |

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10.10.00

* Prefix T is used for Teaching Assessment Number.

** List the amount by weight of the substance used. (for liquids eg; 100 mls 1M Sodium Hydroxide =

4g).

*** Hazdat No is the UNICOSHH datasheet report number.

Biosciences No is the Biosciences data sheet number.

UNICOSHH IS A CHEMICAL DATABASE ON THE HEALTH AND SAFETY UNIT SERVER.

BIOSCIENCES DATA SHEETS ARE AVAILABLE IN THE SCHOOL SAFETY OFFICE.

-----------------------

R. Insall

42507

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