Use of SDS -Page gel for protein.
Acrylamide mix ( Acrylamide and bisacrylamide)
TEMED(N,N,N’,N;-tetramethylethylnediamine)
Sodium Dodecyl sulfate (SDS)Hazardous Substances Policy - Assessment
CHEMICAL HAZARD AND RISK ASSESSMENT
School of Biosciences
|Name of |Dr.Robert Insall | | |Assessment Number* |2032 |
|supervisor | | | | | |
| | Signature | | | |
|Assessor |Soha Zouwail | | |Date of Assessment |17/2/02 |
Signature
Notes A School COSHH form in Word is available on the School Server.
Available from the Health and Safety Unit.
Guidance on making an assessment is given in Making a Chemical Hazard and Risk Assessment.
Guidance is also available from Guidance on Completing the Chemical Hazard and Risk Assessment Form.
Use a continuation sheet to expand any section of this form in hard copy version.
|1 | |LOCATION OF THE WORK ACTIVITY | |8th Floor labs |
| | | | | |
|2 | |PERSONS WHO MAY BE AT RISK |
| | | | |
|List names where possible |Frank Zimmerman,Jason King , Margarita Tchibalina,Robert Insall ,Soha Zouwail, and may be other members of both |
| |Robert’s &Laura’s labs |
| |
| |
|3 | |ACTIVITY ASSESSED | |Use of SDS -Page gel for protein. |
| | | | | |
| | |MATERIALS INVOLVED |Attach copies of data sheet(s) | |
|NAME |AMOUNT** |HAZARD |RISK PHRASES |HAZDAT |BIOSCIENCESNO*** |
| | | | |NO*** | |
|Acrylamide mix |4 ml of 30% Acrylamide|Toxic& |R39/27Very toxic:danger of | | |
|( Acrylamide and bisacrylamide) |mix |Carcinogenic |irreversible effects in contact | | |
| | | |with skin | | |
| | | |R23/24/25 Toxic by inhalation ,in | | |
| | | |contact with the skin &if swallowed| | |
| | | | | | |
| | | |R45 may cause cancer | | |
| | | |R46 may cause heritable genetic | | |
| | | |damage | | |
| | | |R 46 Danger of serious damage to | | |
| | | |health by prolonged exposure | | |
|TEMED(N,N,N’,N;-tetramethylethyl |4 ul |Irritant |R11 highly flammable | | |
|nediamine) | | |R20/22 harmful by inhalation or if | | |
| | | |swallowed | | |
| | | |R34 causes burns | | |
| | | |R 36/37/38 Irritant to eyes | | |
| | | |,respiratory system &skin | | |
|Sodium Dodecyl sulfate (SDS) | 0.1 ml of 10% |Irritant |R41 risk of serious damage to eyes | | |
| |solution |Harmful if swallowed |R42 may cause sensitisation by | | |
| | | |inhalation. | | |
| | | |R20/22 harmful by inhalation &if | | |
| | | |swallowed | | |
| | | |R36/37/38 irritation to eyes, | | |
| | | |respiratory system, &skin. | | |
|Ammonium persulphate (APS) |0.1 ml of 10% APS |Irritant |R8 contact with combustible | | |
| | |Harmful if swallowed |material may cause fire. | | |
| | | |R22 harmful if swallowed. | | |
| | | |R34 causes burns. | | |
| | | |R42/43 may cause sensitisation by | | |
| | | |inhalation or skin contact. | | |
|Hydroxymethylaminomethane(Tris) |2.5 ml of 1.5 M Tris |Irritant |R36/37/38 Irritant to eyes, | | |
| | | |respiratory system, &skin. | | |
|5 | |INTENDED USE |
| | | | |
|Give brief details and attach protocol/instructions |Preparation & use of gels containing different percentages of acrylamide to separate proteins |
| |of different sizes. |
|6 | |RISKS to HEALTH and SAFETY from INTENDED USE |
| | | | |
|From personal exposure or hazardous reactions. Refer to OELs, flash points, etc., as appropriate. Are pregnant women, breast-feeding mothers especially|
|at risk? |
|There is risk of inhalation of powder when preparing SDS solution. There is risk of skin contamination when using acrylamide mix, or skin burn when |
|using the TEMED & APS solutions. |
|7 | |CONCLUSIONS ABOUT RISKS |
| | | | |
|Is level of risk acceptable? Can risk be prevented or reduced by change of substance/procedure? Are control measures necessary? |
|The level of risk is not acceptable and control measures should be followed. |
|8 | |CONTROL MEASURES |
| | | | |
|Additional to Good Chemical Practice | |
|The sodium dodecyl sulphate (SDS) should be weighed in the fume hood on the 7th floor whilst wearing mask, gloves and lab coat. |
| The acrylamide mix &TEMED should be all used while wearing gloves. |
|9 | |INSTRUCTION/TRAINING |
| | | | |
|Specify course(s) and/or special arrangements. | |
|Personnel involved in the clean up procedure must be adequately trained and experienced in handling carcinogenic materials. |
|10 | |MONITORING |
| | | | |
|Performance of control measures, | |
|Check fume hood is working properly before use. |
| |
|Personal exposure | |Health Surveillance | |
|Wearing lab coat and gloves during the whole procedure. | |
| | |
|11 | |WASTE DISPOSAL PROCEDURE |
| See School Server for Approved Procedure Document on specific Chemical Waste Disposal. |
|Polymerised Acrylamide can be placed in yellow bags for incineration. Any of the other chemicals could be disposed in the drain followed by large amount|
|of water. |
|12 | |REVIEW |
| | | | |
|Enter the date or circumstances for review of assessment (maximum review interval 5 years) | |
|February 2007 |
|13 | |EMERGENCY ACTION |
| | | | |
|TO CONTROL HAZARDS |To stabilize situation eg spread absorbant on liquid spill; eliminate sources of ignition, etc. |
|Any spillage of acrylamide should be carefully cleaned whilst wearing gloves and protective clothes and the waste should be discarded as special waste |
|(contact Brian Facer in stores). Treat contaminated surface with household bleach and then wash thoroughly with water. If APS or TEMED has been |
|spilled, sources of ignition and combustible material should be eliminated & absorbant material should be spread on the spill. |
| |
| |
|TO PROTECT PERSONNEL |Evacuation, protection for personnel involved in clean-up, Special First Aid | |
|If any of the hazardous chemicals comes in contact with the skin, remove people from source of contamination, bring injured person under a shower, wash |
|contaminated part of the body for a minimum of 10 minutes, remove any contaminated clothing and seek medical advice. Ensure that the person involved in |
|the clean up is wearing gloves and lab coat. Breathing apparatus may also be required. |
|TO RENDER SITE OF EMERGENCY SAFE |Clean-up/decontamination | |
|For air contamination: evacuate the place and ventilate room as much as possible to dispel vapour. |
|For flammable materials: shut off all sources of ignition in the room, evacuate all people immediately, contact security or ring fire alarm. |
| |
| |
| |
| |
| CONTACT PHONE |
| |
10.10.00
* Prefix T is used for Teaching Assessment Number.
** List the amount by weight of the substance used. (for liquids eg; 100 mls 1M Sodium Hydroxide =
4g).
*** Hazdat No is the UNICOSHH datasheet report number.
Biosciences No is the Biosciences data sheet number.
UNICOSHH IS A CHEMICAL DATABASE ON THE HEALTH AND SAFETY UNIT SERVER.
BIOSCIENCES DATA SHEETS ARE AVAILABLE IN THE SCHOOL SAFETY OFFICE.
-----------------------
R. Insall
42507
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