2.5 C: Photosynthesis Quiz PROCTOR VERSION

2.5 C: Photosynthesis Quiz

PROCTOR VERSION

1. The diagram below shows a partial model of photosystems II and I in the light-dependent

reactions of photosynthesis.

Which statement explains the process that occurs immediately after the electrons are excited in photosystem II? (A) The excited electrons are replaced when an enzyme splits a water molecule into two

electrons, an oxygen atom, and two hydrogen ions. Rationale: This answer suggests the student understands that the excited electrons at the reaction center must be replaced by other electrons, that water is reduced so that the missing electrons at the reaction site can be replaced (the site is a very strong oxidizing agent without electrons), and that no further electron excitation can occur at the reaction center until the missing electrons are replaced. (B) The excited electrons release energy in the form of ATP and are then transported back to the reaction center through a series of redox reactions.

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2.5 C: Photosynthesis Quiz

PROCTOR VERSION

Distractor Rationale:

This answer suggests the student may understand that electrons are transported via a series of redox reactions and that energy is released, but does not understand that the next step after excitation is the splitting of water to replace the excited electrons so that photophosphorylation can continue, that energy is released as electrons are transported through the electron transport chain via a series of redox reactions (and generate ATP via a concentration gradient), or that the electrons are not transported back to the reaction center in photosystem II (noncyclic photophosphorylation).

(C) The excited electrons are accepted by the terminal electron acceptor NADP+ and used to generate ATP.

Distractor Rationale:

This answer suggests the student may understand that electrons are moved through an electron transport chain and ultimately are taken up by the terminal acceptor NADP+, but does not understand that this process is not directly needed to allow for the continued excitation of electrons at the reaction center, and that the replacement of the excited electrons is crucial for the capture of energy from light.

(D) The excited electrons are added to ADP+ to produce ATP, which is used to drive the reactions needed to produce sugar from carbon dioxide in the Calvin cycle.

Distractor Rationale:

This answer suggests the student may understand that ATP is a product of the light reactions, but does not understand that excited electrons at the reaction center must be continually replaced by the oxidation of water molecules in order to capture more energy from photons and provide power for ATP production.

Aligned to: LO 2.5 CA 2.5: Explain Energy Use, Storage & Capture

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2.5 C: Photosynthesis Quiz

PROCTOR VERSION

2. The chloroplasts in a plant cell have a mutation that causes hydrogen ions to diffuse directly

through the phospholipid bilayer of the membranes identified in the diagram below.

Which statement explains how the mutation most directly affects the chloroplasts?

(A) Chlorophyll in the photosystems is not able to use free energy to excite electrons to higher energy levels. Distractor Rationale: This answer suggests the student may understand that chlorophyll uses sunlight to excite electrons, but does not understand that the ability to directly capture energy from sunlight is not affected by this mutation (photosystem II can still occur), and that the mutation does affect the ability to establish an electrochemical gradient, which is necessary to move hydrogen ions into an area of high concentration and allow for ATP production through chemiosmosis.

(B) Enzymes in the stroma are not able to use ATP and NADPH to generate 3-carbon sugars in the Calvin cycle. Distractor Rationale: This answer suggests the student may understand that ATP and NADPH are used to generate 3-carbon sugars in the Calvin cycle and that this occurs in the stroma, but does not understand that these processes are not affected directly by the mutation, and that the ability to establish an electrochemical gradient is affected, which will prevent ATP production via chemiosmosis.

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2.5 C: Photosynthesis Quiz

PROCTOR VERSION

(C) The thylakoid membrane is not able to generate an electrochemical gradient needed for the production of ATP via chemiosmosis. Rationale: This answer suggests the student understands that the mutation affects the cell's ability to produce ATP because hydrogen ions can move freely through the membrane, which prevents the establishment of an electrochemical gradient needed to produce ATP through chemiosmosis.

(D) Enzymes in the thylakoid membrane are not able to split water and generate oxygen through photolysis. Distractor Rationale: This answer suggests the student may understand that water is split by a process that occurs in the thylakoid membrane, but does not understand that the splitting of water is not affected by this mutation and that the ability to establish an electrochemical gradient needed to produce ATP in the light reaction via chemiosmosis is affected.

Aligned to: LO 2.5 CA 2.5: Explain Energy Use, Storage & Capture

3. Noncyclic photophosphorylation and cyclic photophosphorylation are the two pathways that

generate ATP in the light-dependent reactions. The diagrams below show the light-dependent reactions and the Calvin cycle.

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2.5 C: Photosynthesis Quiz

PROCTOR VERSION

Based on the diagrams, which statement best explains why there are two different pathways that generate ATP in the light-dependent reactions? (A) Noncyclic photophosphorylation allows the chloroplast to undergo photosynthesis, even

when no water is available. Distractor Rationale: This answer suggests the student may understand that noncyclic photophosphorylation requires water to donate electrons and cyclic photophosphorylation does not, but does not understand that some water is required for all chemical reactions in cells. (B) The Calvin cycle requires more ATP than NADPH, and the extra ATP is generated by cyclic photophosphorylation without generating an excess of NADPH. Rationale: This answer suggests the student understands that more ATP than NADPH is required by the Calvin cycle and that cyclic photophosphorylation only generates ATP, which helps to prevent an excess of NADPH in the Calvin cycle. (C) Cyclic photophosphorylation requires less energy from photons, which allows ATP to be produced more quickly than in noncyclic photophosphorylation.

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