PDF Six Major Classes of Enzymes and Examples of Their Subclasses

Enzyme definition

Enzymes are protein catalysts that increase the velocity of a chemical reaction and are not consumed during the reaction they catalyze.

[Note: Some types of RNA can act like enzymes, usually catalyzing the cleavage and synthesis of phosphodiester bonds; RNAs with catalytic activity are called ribozymes and are much less commonly encountered than protein catalysts.]

Biologic catalysts

1. Shared properties with chemical catalysts

a. Enzymes are neither consumed nor produced during the course of a reaction.

b.Enzymes do not cause reactions to take place, but they greatly enhance the rate of reactions that would proceed much slower in their absence. They alter the rate but not the equilibrium constants of reactions that they catalyze.

2. Differences between enzymes and chemical catalysts

a. Enzymes are proteins. b. Enzymes are highly specific and produce only the

expected products from the given reactants, or substrates (i.e., there are no side reactions). c. Enzymes may show a high specificity toward one substrate or exhibit a broad specificity, using more than one substrate. d. Enzymes usually function within a moderate pH and temperature range.

Enzymes execute of two basic functions in biological object.

They are catalytic and regulation.

Catalytic efficiency

Most enzyme-catalyzed reactions are highly efficient, proceeding from 103 to 108 times faster than uncatalyzed reactions. Typically, each enzyme molecule is capable of transforming 100 to 1000 substrate molecules into product each second. The number of molecules of substrate converted to product per enzyme molecule per second is called the turnover number.

Regulation

Enzyme activity can be regulated -- that is, enzymes can be activated or inhibited so that the rate of product formation responds to the needs of the cell.

Measures of enzyme activity

1. Specific activity is usually expressed as ?mol of substrate transformed to product per minute per milligram of enzyme under optimal conditions of measurement.

2. Turnover number, or kcal, is the number of substrate molecules metabolized per enzyme molecule per unit time with units of min-1 or s-1.

Enzyme classification

1.Enzymes are divided into six major classes with several subclasses. a. Oxidoreductases are involved in oxidation and reduction. b.Transferases transfer functional groups (e.g., amino or phosphate groups). c. Hydrolases transfer water; that is, they catalyze the hydrolysis of a substrate. d.Lyases add (or remove) the elements of water, ammonia, or carbon dioxide (CO2) to (or from) double bonds. e. Isomerases catalyze rearrangements of atoms within a molecule. f. Ligases join two molecules.

 ................
................

In order to avoid copyright disputes, this page is only a partial summary.

Google Online Preview   Download