Www.thno.org
For immunofluorescence staining, sections were blocked in 10% normal goat serum and incubated with a primary antibody specific for CD31 (#14-0311, 1:100; eBioscience), SIRT1 (#13161-1-AP, 1:50; Proteintech) or α-smooth muscle actin (α-SMA) (#A2547, 1:200; Sigma-Aldrich) followed by an Alexa FluorTM 594 goat anti-rabbit IgG… ................
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