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Antibody titer determination and testosterone determination. The ELISA assay used to determine anti-GnRH antibody concentrations in serum was as follows: 96-well plates were allowed to bind streptavidin at a concentration of 100 ng/well, in carbonate buffer pH 9.6, at room temperature for 3 hours. Plates were then washed 3 times with PBST. ................
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