DNA Technology Worksheet
DNA Technology Worksheet Name:
The picture to the right is an actual electrophoresis gel.
1. What is the appearance of the bands? Are they clear and defined or blurred and indistinguishable?
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2. Which of the labeled bands (A, B, C, etc.) contain the smallest DNA fragments?
**
3. Which of the labeled bands (A, B, C, etc.) contain the largest DNA fragments?
**
4. What is the size of the fragments labeled ‘B? **
5. Did all of these samples come from the same DNA source? **
How do you know?
**
The picture to the right is an actual electrophoresis gel.
6. Lane 1 & 14 are called a standards. What do you notice about the bands produced by the standard?
**
7. A standard consists of DNA fragments that scientists KNOW the lengths of. Why is it important to have a standard?
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8. Did all of these samples come from the same DNA source?
**
How do you know?
**
9. Look at the DNA ‘fingerprints’. Did ANY of the samples come from the same individual? **
How do you know? **
10. Name two situations when DNA fingerprints are useful. **
11. How does the DNA migrate from one end of the gel to the other? **
12. What cuts up the DNA into tiny fragments? **
Continue analyzing DNA profiles on the following page.
Use the gels below to answer the following questions.
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A
B
D
E
C
203
86
52
31
29
S2
felon
S3
S1
Suzy was assaulted in an alley and is a victim of rape. The police collected a sample of sperm that was left at the crime scene and now have 3 suspects in custody. Which of the suspects raped Suzy?
**
dad 1
mom
baby
dad 2
Mrs. Smith has a baby named Jessica. She believes one of two men can be the father of her child. A paternity test is done and the results are shown above. Which of the 2 men are baby Jessica’s father? **
S2
felon
S3
S1
Lt. Russ is investigating a murder scene. The felon was scratched by his victim & some of his skin cells were found under the victim’s fingernails. A DNA test was performed. Which of the suspects is the murderer? **
dad
child
1
mom
child
2
child
3
child
4
child
5
child
6
Mr. & Mrs. Jones just gave birth to fraternal twins- Bob and Jane. Unfortunately, the nurse has confused the Jones twins with 4 other babies. The doctors took samples of DNA from each of the babies and Mr. & Mrs. Jones. Which of the 6 children are Mr. & Mrs. Jones twins? **
mom
son 1
daughter
son 2
The millionaire, Mr. Big, has just died. He has left behind a wife, daughter and a large inheritance. The news of his death has brought forth 2 men who claim to be the long lost son of Mr. & Mrs. Big. Before Mr. & Mrs. Big were married they had an illegitimate child and had placed him up for adoption. They had tried to find him after they became wealthy but had no luck in locating him. A DNA sample was taken from Mrs. Big, the Big daughter and the two men who claim to be the long lost son. Which, if any, of the men are telling the truth? **
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