Sample Biological Material Standard Operating Procedure ...
When expressing proteins (specifically use BL21 E.coli cells), check O.D.600 until it reads between 0.4-0.8, add 100ul 100mM IPTG (inducer). Take 500ul samples at 0h, 4h, 12h, 24h…..(spin down and discard supernatant. Store in -20˚C) to check on SDS-PAGE gel. Day 3: ................
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